Key features and details
- Rabbit polyclonal to Laminin
- Suitable for: Dot blot, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Laminin antibody
See all Laminin primary antibodies
DescriptionRabbit polyclonal to Laminin
SpecificityIn dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C.
Tested applicationsSuitable for: Dot blot, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Dog, Pig, Xenopus laevis, Reptile, Mammals, Amphibian
Full length native protein (purified) corresponding to Laminin. The immunogen is laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.
- IHC-P: Human skin, tongue and tonsil tissue; mouse skin neoplasia tissue.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.027% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab11575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||1/1000. This concentration was determined using laminin at 50 ng per dot.|
|IHC-P||Use at an assay dependent concentration.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityBroadly expressed in: skin, heart, lung, and the reproductive tracts.
Sequence similaritiesContains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain IV is globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- LAMA 1 antibody
- LAMA antibody
- LAMA1 antibody
Immunohistochemical analysis of formalin fixed paraffin-embedded human tongue sections labeling Laminin with ab11575 at 1/30 dilution. Goat Anti-Rabbit IgG, FITC conjugate was used as secondary antibody.
Immunohistochemical analysis of formalin fixed paraffin-embedded human tonsil sections labeling Laminin with ab11575 at 1/50 dilution. Goat Anti-Rabbit IgG, Cy3™ conjugate was used as secondary antibody.
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane was separated on SDS-PAGE and probed with Rabbit Anti-Laminin ab11575. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase secondary antibody and a chemiluminescent substrate.
1. antibody dilution 1/1,000
2. antibody dilution 1/2,000
3. antibody dilution 1/4,000
4. antibody dilution 1/8,000
5. antibody dilution 1/16,000
6. antibody dilution 1/32,000
7. Negative control: without primary antibody
Cellular and embryonic localisation of CL-L1
Immunofluorescence showing co-localisation of CL-L1 and Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium and in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm.
(After Fugure 2 E of Munye et al)
This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.
ab11575 staining Laminin in mouse oocytes by Immunohistochemistry (PFA fixed, paraffin embedded sections).
Grafts were fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned in 5 to 8 µm intervals. In brief, sections on slides were de-paraffinized, re-hydrated, antigens unmasked by incubating in target retrieval solution at 95°C for 30 minutes, permeabilized in 0.1% Triton-X100 for 5 minutes, blocked with 10% chicken serum in TBST overnight, and incubated with primary antibody at 1/200, in TBST with 1% serum for 1 hour at room temperature. After washing in TBST, slides were incubated with secondary antibody at 1/1000, for 30 minutes at room temperature. Cover slips were mounted with Prolong Gold Antifade with DAPI.
Immunofluorescence revealed TRA98+ oocytes and FOXL2+ granulosa cells in ovarian cord-like structures (dashed lines) and Laminin+ basement membrane (red) following five days of intact e12.5 female genital ridge transplantation. Alo
ab11575 staining Laminin in Mouse skin neoplasia - papilloma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/200) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
ab11575 has been referenced in 341 publications.
- Marsala M et al. Spinal parenchymal occupation by neural stem cells after subpial delivery in adult immunodeficient rats. Stem Cells Transl Med 9:177-188 (2020). PubMed: 31800978
- Molè MA et al. Integrin-Mediated Focal Anchorage Drives Epithelial Zippering during Mouse Neural Tube Closure. Dev Cell 52:321-334.e6 (2020). PubMed: 32049039
- Miranda CMFC et al. Decellularization of Extracellular Matrix from Equine Skeletal Muscle. J Equine Vet Sci 90:102962 (2020). PubMed: 32534761
- Chow T et al. Recapitulating kidney development in vitro by priming and differentiating mouse embryonic stem cells in monolayers. NPJ Regen Med 5:7 (2020). PubMed: 32351711
- Huang CW et al. FGF9 induces functional differentiation to Schwann cells from human adipose derived stem cells. Theranostics 10:2817-2831 (2020). PubMed: 32194837