Product nameAnti-Laminin antibody
See all Laminin primary antibodies
DescriptionRabbit polyclonal to Laminin
Tested applicationsSuitable for: RIA, Sandwich ELISA, ICC/IF, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Purified laminin P1 fragment from human placenta.
- Human skin
This antibody primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Concentration information loading...
PurityIon Exchange Chromatography
Primary antibody notesThis antibody primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion.
Our Abpromise guarantee covers the use of ab23753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 5 µg/ml.
For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab49726 as Detection.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/600.
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase in TBS 15 min at 37°C. There after, non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
|ELISA||Use at an assay dependent concentration.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityBroadly expressed in: skin, heart, lung, and the reproductive tracts.
Sequence similaritiesContains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain IV is globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
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ab23753 has not yet been referenced specifically in any publications.