Product nameAnti-Laminin antibody
See all Laminin primary antibodies
DescriptionRabbit polyclonal to Laminin
Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Mammals
Full length native protein isolated from mouse EHS tumor(purified)
- This antibody gave a positive signal in MEF1 cell line in IF/ICC.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituent: 50% Glycerol
Our Abpromise guarantee covers the use of ab30320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 337 kDa.|
|ICC/IF||1/100 - 1/2500.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityBroadly expressed in: skin, heart, lung, and the reproductive tracts.
Sequence similaritiesContains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain IV is globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- LAMA 1 antibody
- LAMA antibody
- LAMA1 antibody
Rat peripheral nerve (low and high magnification; formalin fixed, frozen sections). Immuno-FITC stain (color inverted) with 1:1000 anti-Laminin1 antibody (ab30320).
ICC/IF image of ab30320 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30320, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Gluck JM et al. Biochemical and biomechanical properties of the pacemaking sinoatrial node extracellular matrix are distinct from contractile left ventricular matrix. PLoS One 12:e0185125 (2017). Read more (PubMed: 28934329) »
- Hu L et al. Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration. Biomed Res Int 2017:9342714 (2017). Read more (PubMed: 29387727) »