Anti-Laminin beta 1 antibody [DG10] (ab88279)
Key features and details
- Mouse monoclonal [DG10] to Laminin beta 1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Laminin beta 1 antibody [DG10]
See all Laminin beta 1 primary antibodies -
Description
Mouse monoclonal [DG10] to Laminin beta 1 -
Host species
Mouse -
Specificity
ab88279 appears to react with the NH2 terminus of Laminin beta 1. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Laminin beta 1. Partially pepsin digested Laminins from human placenta.
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Positive control
- IHC-P: Fetal kidney
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General notes
ab88279 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
ab88279 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. -
Clonality
Monoclonal -
Clone number
DG10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Sequence similarities
Contains 13 laminin EGF-like domains.
Contains 1 laminin IV type B domain.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Major component. - Information by UniProt
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Database links
- Entrez Gene: 3912 Human
- Omim: 150240 Human
- SwissProt: P07942 Human
- Unigene: 650585 Human
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Alternative names
- CLM antibody
- Cutis laxa with marfanoid phenotype antibody
- LAM B1 antibody
see all
Images
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Immunohistochemical analysis of formalin fixed, parafin embedded fetal kidney tissue labelling Laminin beta with ab98597 at 1/50 dilution.
Pretreated with EDTA.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab88279 has not yet been referenced specifically in any publications.