Product nameAnti-Laminin beta 1 antibody [EPR3190(2)]
See all Laminin beta 1 primary antibodies
DescriptionRabbit monoclonal [EPR3190(2)] to Laminin beta 1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Laminin beta 1 aa 1700-1800. The exact sequence is proprietary.
- Human fetal kidney and A431 lysates, A431 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 198 kDa).|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Sequence similaritiesContains 13 laminin EGF-like domains.
Contains 1 laminin IV type B domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Major component.
- Information by UniProt
- CLM antibody
- Cutis laxa with marfanoid phenotype antibody
- LAM B1 antibody
All lanes : Anti-Laminin beta 1 antibody [EPR3190(2)] (ab109293) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A431 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 198 kDa
Flow cytometric analysis of permeabilized A431 cells using ab109293 (red) at a diluiton of 1/100 or a rabbit IgG (negative) (green).
This product has been referenced in:
- Xu S et al. Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets. Exp Ther Med 18:1099-1106 (2019). Read more (PubMed: 31316605) »
- Weng S et al. Role of quercetin in protecting ARPE-19 cells against H2O2-induced injury via nuclear factor erythroid 2 like 2 pathway activation and endoplasmic reticulum stress inhibition. Mol Med Rep 16:3461-3468 (2017). Read more (PubMed: 28713895) »