Overview

  • Product name

    Anti-Laminin beta 1 antibody [LT3]
    See all Laminin beta 1 primary antibodies
  • Description

    Rat monoclonal [LT3] to Laminin beta 1
  • Host species

    Rat
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Fr, IHC (Methanol fixed), IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Hamster, Human, Pig
  • Immunogen

    Murine EHS laminin preparation.

  • Positive control

    • LS174T cells. Normal colon or colon carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab44941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/200. Detects a band of approximately 210 kDa.
IP 1/400.
IHC-Fr 1/400. Use with acetone fixed tissues.
IHC (Methanol fixed) 1/400.
IHC-P Use at an assay dependent dilution.

Target

  • Function

    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Sequence similarities

    Contains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • Domain

    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • Database links

  • Alternative names

    • CLM antibody
    • Cutis laxa with marfanoid phenotype antibody
    • LAM B1 antibody
    • LAMB 1 antibody
    • LAMB1 antibody
    • LAMB1_HUMAN antibody
    • Laminin B1 antibody
    • Laminin B1 chain antibody
    • Laminin beta 1 chain antibody
    • Laminin beta 1 chain precursor antibody
    • Laminin beta1 antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit beta-1 antibody
    • Laminin-1 subunit beta antibody
    • Laminin-10 subunit beta antibody
    • Laminin-12 subunit beta antibody
    • Laminin-2 subunit beta antibody
    • Laminin-6 subunit beta antibody
    • Laminin-8 subunit beta antibody
    • LIS5 antibody
    • MGC142015 antibody
    see all

Images

  • ICC/IF image of ab44941 staining human prostate cells.  The cells were fixed with 4% paraformaldehyde, permeabilised with 0.8% Triton X-100, and blocked with 20% serum for 1 hour at 24°C.  The primary anitibody was diluted 1/400 in in 20% horse serum in PBS and incubated for 20 minutes at 4°C.  An Alexa Fluor® 488 conjugated donkey anti-rat was used as the secondary antibody.

    The blue staining is the cell nuclei, the green staining shows the laminin beta 1.

    See Abreview

  • ab44941 staining Laminin beta 1 in porcine jejunum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol/acetone, permeabilized using 0.3% Triton, blocked with 1% normal goat serum for 10 minutes at room temperature and then incubated with ab44941 at a 1/800 dilution for 2 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rat IgG (H+L) used at a 1/200 dilution.

    See Abreview

References

This product has been referenced in:

  • Shirali AS  et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). Read more (PubMed: 29615716) »
  • Billin AN  et al. HIF prolyl hydroxylase inhibition protects skeletal muscle from eccentric contraction-induced injury. Skelet Muscle 8:35 (2018). Read more (PubMed: 30424786) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Melanoma cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Melanoma cell lines
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 24 2017

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (melanoma cell line)
Total protein in input
1000 µg
Immuno-precipitation step
Protein G
Specification
melanoma cell line

Abcam user community

Verified customer

Submitted Nov 18 2015

Question
Answer

Thank you for that information.

I have gone ahead and set up a credit for the cost of your original order. Once we recieve the 3 vials of unopened antibody from you the credit will be applied.

When you return the goods, please ensure that you follow the procedures as below:

- Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
- Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet)
- Please return via an overnight courier such as UPS, DHL, or FEDEX.
- Ship to the following address:

Attn: Returns ORDER REF: (*)
Abcam Inc
One Kendall Square
Bldg 200, Suite 341
Cambridge, MA 02139


Please be advised that a 20% restock fee will apply to this order. The most up-to-date information regarding your order, invoice, and credit note status is available online in your Abcam account information. If your order was placed by credit card, your refund will be issued to your credit card. If your order was placed by Purchase Order, all customers must submit a credit note with their invoice to their Accounts Payable department to be handled. The Accounts Payable must have a copy of the credit note so the original invoice can be updated to reflect the correct amount owed.

If you have any other questions please do not hesitate to contact us!

Email: US.orders@Abcam.com
Phone: 888-77-ABCAM(22226)
Fax: 866 739 9884
Customer Service hours of operation:
Monday-Friday 8:30 AM- 9:00PM EST

Read More

Answer

Thank you for your reply and for providing that information.

I just had a few more follow up questions:

1 - You received 4 vials of ab44941. How many of the bottles did you test? I ask incase the issue may be limited to a specific bottle.

2 - You used Low pH antigen retrieval methods for ab44941, but did you also try the High pH antigen retrieval? If so, were the results the same?

I look forward to your reply and resolving this issue.

Read More

Answer

Thank you for contacting Abcam.

I am sorry that you are experiencing some issues with ab44941. The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on Mouse, Hamster, Human & Pig samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

To be able to help you further in this matter, would you please be able to let me know what type of samples you are using and also providea copy of the protocol you had been using (including any variations in antigen retrieval and primary antibody concentrations)?

I look forward to your reply and helping to resolve this issue. If you could also provide either the Abcam order number or the PO# used to purchase the antibody, that would be very helpful.

Read More

Answer

Thank you for your inquiry.


When conducting IHC or IF, it is generally not advisable to use antibodies which are raised in the same species as the target sample (e.g. a mouse monoclonal antibody against mouse samples). The secondary antibody may recognize endogenous IgG in the sample tissue as well as the primary antibody, which may lead to non-specific staining and high background.

At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund. While we do not other available information for this in mouse with these applications. These are 100% covered by the Abpromise.

In this instance however you should not have any issues with cross reactivity as the species IgG differences are great enough. However in order to assure limited cross reactivity I would recommend using a pre-absorbed secondary antibody. This means they have been absorbed with other proteins, or serum from variousspecies, to eliminate any antibody that may cross-react. The resulting purified antibody should be very pure and specific and any crossreactivity should be significantly reduced. We do offer a number of fluorescently tagged anti-rat secondary antibodies which are pre-absorbs such as ab98386 (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-DyLight-488-pre-adsorbed-ab98386.html)and ab98387(https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-DyLight-550-pre-adsorbed-ab98387.html)



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (small intestine (jejunum))
Specification
small intestine (jejunum)
Fixative
Methanol/Acetone
Permeabilization
Yes - 0.3% Triton
Blocking step
(agent) for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 02 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate)
Specification
prostate
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton-X
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 14 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.8% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jan 07 2008

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