Product nameAnti-Laminin beta 1 antibody [LT3]
See all Laminin beta 1 primary antibodies
DescriptionRat monoclonal [LT3] to Laminin beta 1
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Fr, IHC (Methanol fixed), IHC-Pmore details
Species reactivityReacts with: Mouse, Hamster, Human, Pig
Murine EHS laminin preparation.
- LS174T cells. Normal colon or colon carcinoma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab44941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/200. Detects a band of approximately 210 kDa.|
|IHC-Fr||1/400. Use with acetone fixed tissues.|
|IHC (Methanol fixed)||1/400.|
|IHC-P||Use at an assay dependent dilution.|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Sequence similaritiesContains 13 laminin EGF-like domains.
Contains 1 laminin IV type B domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Major component.
- Information by UniProt
- CLM antibody
- Cutis laxa with marfanoid phenotype antibody
- LAM B1 antibody
ICC/IF image of ab44941 staining human prostate cells. The cells were fixed with 4% paraformaldehyde, permeabilised with 0.8% Triton X-100, and blocked with 20% serum for 1 hour at 24°C. The primary anitibody was diluted 1/400 in in 20% horse serum in PBS and incubated for 20 minutes at 4°C. An Alexa Fluor® 488 conjugated donkey anti-rat was used as the secondary antibody.
The blue staining is the cell nuclei, the green staining shows the laminin beta 1.
ab44941 staining Laminin beta 1 in porcine jejunum tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in methanol/acetone, permeabilized using 0.3% Triton, blocked with 1% normal goat serum for 10 minutes at room temperature and then incubated with ab44941 at a 1/800 dilution for 2 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rat IgG (H+L) used at a 1/200 dilution.
This product has been referenced in:
- Shirali AS et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). Read more (PubMed: 29615716) »
- Billin AN et al. HIF prolyl hydroxylase inhibition protects skeletal muscle from eccentric contraction-induced injury. Skelet Muscle 8:35 (2018). Read more (PubMed: 30424786) »