Overview

  • Product name

    Anti-Laminin gamma 1 antibody [A5] - BSA and Azide free
    See all Laminin gamma 1 primary antibodies
  • Description

    Rat monoclonal [A5] to Laminin gamma 1 - BSA and Azide free
  • Host species

    Rat
  • Specificity

    This antibody uniformly stains all human and murine basement membranes.
  • Tested applications

    Suitable for: IP, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Murine EHS laminin preparation.

  • Positive control

    • LS174T cells. Normal colon or colon carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab80580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
IHC-P
IHC-Fr
ICC/IF
  • Application notes
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 µg/ml.
    IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml.
    IP: Use at 2µg/mg of lysate. (Native and denatured)

    Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificity

      Found in the basement membranes (major component).
    • Sequence similarities

      Contains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • Domain

      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    Images

    • Frozen human tonsil stained with ab80580 at 2µg/ml using peroxidase-conjugate and DAB chromogen. Note basement membrane and cell surface staining of blood vessels.
    • Native IP using LS174T cells:
      IP 2µg/mg lysate.
      WB at 2µg/ml.

    References

    This product has been referenced in:

    • Smolders J  et al. Tissue-resident memory T cells populate the human brain. Nat Commun 9:4593 (2018). IHC-P ; Human . Read more (PubMed: 30389931) »
    • Calyjur PC  et al. The mdx Mutation in the 129/Sv Background Results in a Milder Phenotype: Transcriptome Comparative Analysis Searching for the Protective Factors. PLoS One 11:e0150748 (2016). IHC . Read more (PubMed: 26954670) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us. We can recommend using antibody ab106783, ab99667, ab99666 etc. Rabbit or goat anti rat IgG also cross reacts with IgG2a so anti rat IgGs can also be used. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up