Product nameAnti-Laminin gamma 1 antibody [A5] - BSA and Azide free
See all Laminin gamma 1 primary antibodies
DescriptionRat monoclonal [A5] to Laminin gamma 1 - BSA and Azide free
SpecificityThis antibody uniformly stains all human and murine basement membranes.
Tested applicationsSuitable for: IP, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Murine EHS laminin preparation.
- LS174T cells. Normal colon or colon carcinoma.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesPurified from ascites.
Light chain typekappa
Our Abpromise guarantee covers the use of ab80580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at a concentration of 2 µg/ml.
IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml.
IP: Use at 2µg/mg of lysate. (Native and denatured)
Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityFound in the basement membranes (major component).
Sequence similaritiesContains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- LAMB2 antibody
- lamc1 antibody
- LAMC1_HUMAN antibody
This product has been referenced in:
- Smolders J et al. Tissue-resident memory T cells populate the human brain. Nat Commun 9:4593 (2018). IHC-P ; Human . Read more (PubMed: 30389931) »
- Calyjur PC et al. The mdx Mutation in the 129/Sv Background Results in a Milder Phenotype: Transcriptome Comparative Analysis Searching for the Protective Factors. PLoS One 11:e0150748 (2016). IHC . Read more (PubMed: 26954670) »