Product nameAnti-Laminin gamma 1 antibody
See all Laminin gamma 1 primary antibodies
DescriptionRabbit polyclonal to Laminin gamma 1
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized peptide derived from the internal region of human Laminin gamma 1.
- Extracts from HUVEC cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately >170 kDa (predicted molecular weight: 178 kDa).|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityFound in the basement membranes (major component).
Sequence similaritiesContains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- LAMB2 antibody
- lamc1 antibody
- LAMC1_HUMAN antibody
All lanes : Anti-Laminin gamma 1 antibody (ab69632) at 1/500 dilution
Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 178 kDa
Observed band size: >170 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Yan Y et al. Laminins in an in vitro anterior lens capsule model established using HLE B-3 cells. Mol Med Rep 17:5726-5733 (2018). Read more (PubMed: 29436687) »
- Wei X et al. Kojic acid inhibits senescence of human corneal endothelial cells via NF-?B and p21 signaling pathways. Exp Eye Res 180:174-183 (2018). Read more (PubMed: 30597146) »