Key features and details
- Rabbit polyclonal to Laminin gamma 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Laminin gamma 1 antibody
See all Laminin gamma 1 primary antibodies
DescriptionRabbit polyclonal to Laminin gamma 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized peptide derived from the internal region of human Laminin gamma 1.
- Extracts from HUVEC cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately >170 kDa (predicted molecular weight: 178 kDa).|
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityFound in the basement membranes (major component).
Sequence similaritiesContains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- LAMB2 antibody
- lamc1 antibody
- LAMC1_HUMAN antibody
All lanes : Anti-Laminin gamma 1 antibody (ab69632) at 1/500 dilution
Lane 1 : extracts from HUVEC cells
Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 178 kDa
Observed band size: >170 kDa why is the actual band size different from the predicted?
ab69632 has been referenced in 4 publications.
- Yan Y et al. Laminin a4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract. Aging (Albany NY) 11:2699-2723 (2019). PubMed: 31076560
- Yan Y et al. Laminins in an in vitro anterior lens capsule model established using HLE B-3 cells. Mol Med Rep 17:5726-5733 (2018). PubMed: 29436687
- Wei X et al. Kojic acid inhibits senescence of human corneal endothelial cells via NF-?B and p21 signaling pathways. Exp Eye Res 180:174-183 (2018). PubMed: 30597146
- Little GH et al. Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion. Nucleic Acids Res 40:3538-47 (2012). WB ; Human . PubMed: 22187159