Recombinant Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)
![Western blot - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)](https://www.abcam.com/ps/products/233/ab233390/Images/ab233390-1-233390WB1.jpg "Western blot - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21200] to Laminin gamma 1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Laminin gamma 1 antibody [EPR21200]
See all Laminin gamma 1 primary antibodies -
Description
Rabbit monoclonal [EPR21200] to Laminin gamma 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Laminin gamma 1 aa 1400 to the C-terminus. The exact sequence is proprietary.
Database link: P11047 -
Positive control
- WB: Human fetal kidney and fetal heart tissue lysate. A431, OVCAR-3, JAR, MCF7 and A549 whole cell lysate. IHC-P: Human skin, meningioma and kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21200 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab233390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 178 kDa. | |
| IHC-P | 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Found in the basement membranes (major component). -
Sequence similarities
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 3915 Human
- Omim: 150290 Human
- SwissProt: P11047 Human
- SwissProt: Q5VYE7 Human
- SwissProt: Q6NVY8 Human
- Unigene: 609663 Human
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Alternative names
- LAMB2 antibody
- lamc1 antibody
- LAMC1_HUMAN antibody
see all
Images
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Western blot - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)All lanes : Anti-Laminin gamma 1 antibody [EPR21200] (ab233390) at 1/5000 dilution
Lane 1 : Human fetal heart tissue lysate at 10 µg
Lane 2 : Human fetal kidney tissue lysate at 10 µg
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate at 10 µg
Lane 4 : NIH:OVCAR-3 (Human ovary adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 5 : JAR (human placenta choriocarcinoma cell line) whole cell lysate at 20 µg
Lane 6 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate at 20 µg
Secondary
Lanes 1-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 5-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 178 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 10 seconds; Lanes 2-4: 15 seconds; Lane 5: 26 seconds; Lanes 6-7: 6 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 18263645, 11067943, 23857767, 28928891).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)](https://www.abcam.com/ps/products/233/ab233390/Images/ab233390-4-233390IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)Immunohistochemical analysis of paraffin-embedded human meningioma tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in human meningioma (PMID: 23053286) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)](https://www.abcam.com/ps/products/233/ab233390/Images/ab233390-3-233390IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human kidney (PMID: 21903675) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)](https://www.abcam.com/ps/products/233/ab233390/Images/ab233390-2-233390IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human skin (PMID: 15159456) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
![Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)](https://www.abcam.com/ps/products/233/ab233390/Images/ab233390-5-benefits-of-recombinant-antibodies.png)
Anti-Laminin gamma 1 antibody [EPR21200] (ab233390)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab233390 has not yet been referenced specifically in any publications.
