Overview

  • Product name

    Anti-Laminin gamma 1 antibody [EPR8217]
    See all Laminin gamma 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8217] to Laminin gamma 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Laminin gamma 1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, JAR and A431 cell lysates; Permeabilized A431 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 178 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificity

      Found in the basement membranes (major component).
    • Sequence similarities

      Contains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • Domain

      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    Images

    • All lanes : Anti-Laminin gamma 1 antibody [EPR8217] (ab134059) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 178 kDa

    • Flow Cytometry analysis of permeabilized A431 cells labelling Laminin gamma 1 with ab134059 at 1/10 dilution (red). Rabbit IgG negative control shown in green.

    References

    This product has been referenced in:

    • Andrews MC  et al. Systems analysis identifies miR-29b regulation of invasiveness in melanoma. Mol Cancer 15:72 (2016). WB ; Human . Read more (PubMed: 27852308) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134059.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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