Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8217] to Laminin gamma 1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-Laminin gamma 1 antibody [EPR8217]
See all Laminin gamma 1 primary antibodies
DescriptionRabbit monoclonal [EPR8217] to Laminin gamma 1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Laminin gamma 1 aa 1-100 (N terminal). The exact sequence is proprietary.
- HeLa, JAR and A431 cell lysates; Permeabilized A431 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 178 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityFound in the basement membranes (major component).
Sequence similaritiesContains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI and IV are globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- LAMB2 antibody
- lamc1 antibody
- LAMC1_HUMAN antibody
All lanes : Anti-Laminin gamma 1 antibody [EPR8217] (ab134059) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 178 kDa
Flow Cytometry analysis of permeabilized A431 cells labelling Laminin gamma 1 with ab134059 at 1/10 dilution (red). Rabbit IgG negative control shown in green.
ab134059 has been referenced in 1 publication.
- Andrews MC et al. Systems analysis identifies miR-29b regulation of invasiveness in melanoma. Mol Cancer 15:72 (2016). WB ; Human . PubMed: 27852308