Recombinant
RabMAb

Recombinant Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)

Overview

  • Product name

    Anti-Laminin gamma 3 antibody [EPR22699-151]
    See all Laminin gamma 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR22699-151] to Laminin gamma 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse Laminin aa 50-300. The exact sequence is proprietary.
    Database link: Q9R0B6

  • Positive control

    • WB: Mouse testis and Mouse kidney lysates. IHC-P: Mouse testis, Mouse cerebrum and Mouse lung tissues. FC: RAW 264.7 cells. IHC-fr: Mouse testis tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
IHC-P 1/500.
WB 1/1000. Predicted molecular weight: 171 kDa.
Flow Cyt 1/50.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificity

      Strongly expressed in capillaries and arterioles of kidney as well as in interstitial Leydig cells of testis.
    • Sequence similarities

      Contains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • Domain

      The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domain IV is globular.
    • Cellular localization

      Secreted, extracellular space, extracellular matrix, basement membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Lamc3 antibody
      • LAMC3_MOUSE antibody
      • Laminin subunit gamma-3 antibody
      • Laminin-12 subunit gamma antibody
      • Laminin-14 subunit gamma antibody
      • Laminin-15 subunit gamma antibody
      see all

    Images

    • All lanes : Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429) at 1/10000 dilution

      Lane 1 : Mouse testis tissue lysate 20 µg
      Lane 2 : Mouse kidney tissue lysate 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 171 kDa
      Observed band size: 172 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST   Exposure time: 3 minutes 

    • Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling Laminin gamma 3 with Ab234429 at 1/100 (4.99 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    • Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Laminin gamma 3 with ab234429 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    • Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Laminin gamma 3 with ab234429 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the basement membrane in the mouse lung is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Laminin gamma 3 with ab234429 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the blood vessels in the mouse cerebrum (PMID:15824114) is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Laminin gamma 3 with ab234429 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the basement membrane in the mouse testis (PMID: 15824114) is observed. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    References

    ab234429 has not yet been referenced specifically in any publications.

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