Anti-LAMP1 antibody [1D4B] (ab25245)
- Datasheet
- References (73)
- Protocols
Overview
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Product name
Anti-LAMP1 antibody [1D4B]
See all LAMP1 primary antibodies -
Description
Rat monoclonal [1D4B] to LAMP1 -
Host species
Rat -
Specificity
Murine CD107a/LAMP1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa) -
Tested applications
Suitable for: IHC-P, ICC/IF, Other, Flow Cyt, IP, IHC-Fr, WB, Immunomicroscopymore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Plasma membrane fraction of mouse embryo NIH3T3 cell line.
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Positive control
- IHC-P: human lung FFPE tissue sections
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
1D4B -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab25245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 17215451 | |
| Other | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| AP | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa). | |
| Immunomicroscopy | Use at an assay dependent concentration. |
Target
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Function
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 3916 Human
- Entrez Gene: 16783 Mouse
- Omim: 153330 Human
- SwissProt: P11279 Human
- SwissProt: P11438 Mouse
- Unigene: 494419 Human
- Unigene: 16716 Mouse
- Unigene: 475822 Mouse
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Alternative names
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [1D4B] (ab25245)](https://www.abcam.com/ps/products/25/ab25245/Images/ab25245-35882-anti-lamp1-antibody-1d4b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [1D4B] (ab25245)This image is courtesy of an anonymous Abreviewab25245 staining LAMP1 in Mouse epithelial cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with methanol/acetone 1:1 and blocked with 1% milk in PNB for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) in PNB for 16 hours at 4°C. An Alexa Fluor® 568-conjugated Donkey polyclonal to rat IgG, dilution 1/500, was used as secondary antibody. Nuclei were stained blue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1D4B] (ab25245)](https://www.abcam.com/ps/products/25/ab25245/Images/ab25245-35880-anti-lamp1-antibody-1d4b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [1D4B] (ab25245)IHC image of LAMP1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25245, 10µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-LAMP1 antibody [1D4B] (ab25245)](https://www.abcam.com/ps/products/25/ab25245/Images/ab25245-35881-anti-lamp1-antibody-1d4b-flow-cytometry.jpg)
Flow Cytometry - Anti-LAMP1 antibody [1D4B] (ab25245)Overlay histogram showing Jurkat cells stained with ab25245 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25245, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions. -
![Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [1D4B] (ab25245)](https://www.abcam.com/ps/products/25/ab25245/Images/ab25245-35883-anti-lamp1-antibody-1d4b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [1D4B] (ab25245)This image is courtesy of an Abreview submitted by Dr Tian Xiaab25245 staining mouse macrophage, hepatoma cell by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 594 conjugated goat anti-mouse antibody was used as the secondary.
Protocols
References
This product has been referenced in:
- Li F et al. HSPB8 over-expression prevents disruption of blood-brain barrier by promoting autophagic flux after cerebral ischemia/reperfusion injury. J Neurochem 148:97-113 (2019). Read more (PubMed: 30422312) »
- Kim BR et al. Phagosome Escape of Rough Mycobacterium abscessus Strains in Murine Macrophage via Phagosomal Rupture Can Lead to Type I Interferon Production and Their Cell-To-Cell Spread. Front Immunol 10:125 (2019). Read more (PubMed: 30766538) »
