Anti-LAMP1 antibody (ab62562)

Rabbit polyclonal LAMP1 antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 8 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name

  • Description

    Rabbit polyclonal to LAMP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 15 amino acid peptide from near the centre of human LAMP1.

  • Positive control

    • EL4 cell lysate. Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • Sequence similarities

    Belongs to the LAMP family.
  • Post-translational
    modifications

    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • Cellular localization

    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Images

  • Immunofluorescence of LAMP-1 in Human Colon cells using ab62562 at 20 ug/mL.

  • Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/ml
    Lane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/ml

    All lanes : EL4 cell lysate

    Predicted band size: 45 kDa

  • Immunohistochemistry of LAMP-1 in rat colon tissue with ab62562 at 2.5 µg/ml.

  • Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requi

References

This product has been referenced in:

  • Sinkevicius KW  et al. RNaseT2 knockout rats exhibit hippocampal neuropathology and deficits in memory. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29752287) »
  • Hung COY & Livesey FJ Altered ?-Secretase Processing of APP Disrupts Lysosome and Autophagosome Function in Monogenic Alzheimer's Disease. Cell Rep 25:3647-3660.e2 (2018). Read more (PubMed: 30590039) »
See all 10 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100 in TBS for 10 minutes
Specification
mouse hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Feb 14 2017

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