RecombinantRabMAb

Recombinant Anti-LAMP1 antibody [EPR21026] (ab208943)

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Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [EPR21026] (ab208943)
  • Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [EPR21026] (ab208943)
  • Western blot - Anti-LAMP1 antibody [EPR21026] (ab208943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
  • Immunohistochemistry (Frozen sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
  • Western blot - Anti-LAMP1 antibody [EPR21026] (ab208943)
  • Immunoprecipitation - Anti-LAMP1 antibody [EPR21026] (ab208943)
  • Anti-LAMP1 antibody [EPR21026] (ab208943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21026] to LAMP1
  • Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-LAMP1 antibody [EPR21026]
    See all LAMP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21026] to LAMP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung, colon, kidney and liver lysates; Neuro-2a, RAW 264.7 and NIH/3T3 whole cell lysates. IHC-P: Mouse spleen and lung tissues. IHC-Fr: Mouse kidney tissue. ICC/IF: NIH/3T3 and Neuro-2a cells. Flow Cyt (intra): Neuro-2a cells. IP: NIH/3T3 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab208943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/100.

Perform heat mediated antigen retrieval using sodium citrate buffer (pH 6.0).
IP
1/30.
ICC/IF (1)
1/100.
Flow Cyt (Intra)
1/500.
Notes
WB
1/1000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/100.

Perform heat mediated antigen retrieval using sodium citrate buffer (pH 6.0).
IP
1/30.
ICC/IF
1/100.
Flow Cyt (Intra)
1/500.

Target

  • Function

    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.

  • Sequence similarities

    Belongs to the LAMP family.

  • Post-translational
    modifications

    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.

  • Cellular localization

    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD107 antigen like family member A antibody
      • CD107 antigen-like family member A antibody
      • CD107a antibody
      • CD107a antigen antibody
      • LAMP 1 antibody
      • LAMP-1 antibody
      • LAMP1 antibody
      • LAMP1_HUMAN antibody
      • LAMPA antibody
      • LGP120 antibody
      • lgpA antibody
      • Lysosomal membrane glycoprotein 120KD antibody
      • Lysosomal Associated Membrane Protein 1 antibody
      • Lysosome associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane protein 1 antibody
      • OTTHUMP00000040663 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [EPR21026] (ab208943)

      Immunofluorescent analysis of 100% methanol-fixed Neuro-2a (mouse neuroblastoma cell line) cells labeling LAMP1 with ab208943 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889), at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [EPR21026] (ab208943)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methonol-permeabilized Neuro-2a (mouse neuroblastoma cell line) cell line labeling LAMP1 with ab208943 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Western blot - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Western blot - Anti-LAMP1 antibody [EPR21026] (ab208943)
      All lanes : Anti-LAMP1 antibody [EPR21026] (ab208943) at 1/2000 dilution

      Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg
      Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
      Lane 3 : Mouse kidney lysate at 20 µg
      Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 43 kDa
      Observed band size: 90-120 kDa why is the actual band size different from the predicted?



      Exposure time : Lanes 1-2: 3 minutes; Lane 3: 5 seconds; Lane 4: 10 seconds.

      Blocking and dilution buffer: 5% NFDM/TBST.

       

      The varying band sizes are due to different levels of glycosylation (PMID: 10212251, PMID: 26246576).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling LAMP1 with ab208943 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse spleen was observed, performed on a Leica Biosystems BOND® RX instrument (PMID: 22008915). Counter stained with hematoxylin.
       
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
       
      Note: BOND® is a registered trademark of Leica Biosystems Melbourne Pty Ltd.
      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling LAMP1 with ab208943 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line.
       
      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889), at 1/200 dilution (red).
       
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling LAMP1 with ab208943 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse spleen was observed, performed on a Leica Biosystems BOND® RX instrument (PMID: 22008915). Counter stained with hematoxylin.
       
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
       
      Note: BOND® is a registered trademark of Leica Biosystems Melbourne Pty Ltd.
      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Frozen sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunohistochemistry (Frozen sections) - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse kidney tissue labeling LAMP1 with ab208943 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining in the endothelial cells of glomeruli and epithelial cells of renal tubules (PMID: 23229015; PMID:23635510). The nuclear counter stain is DAPI (blue).
       
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
    • Western blot - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Western blot - Anti-LAMP1 antibody [EPR21026] (ab208943)
      All lanes : Anti-LAMP1 antibody [EPR21026] (ab208943) at 1/1000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Mouse colon lysate
      Lane 3 : Mouse liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Predicted band size: 43 kDa
      Observed band size: 90-120 kDa why is the actual band size different from the predicted?




      Exposure time : Lanes 1-2: 15 seconds; Lane 3: 5 seconds.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-LAMP1 antibody [EPR21026] (ab208943)
      Immunoprecipitation - Anti-LAMP1 antibody [EPR21026] (ab208943)

      LAMP1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab208943 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208943 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).

      Lane 2: ab208943 IP in NIH/3T3 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208943 in NIH/3T3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Anti-LAMP1 antibody [EPR21026] (ab208943)
      Anti-LAMP1 antibody [EPR21026] (ab208943)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (8)

    Publishing research using ab208943? Please let us know so that we can cite the reference in this datasheet.

    ab208943 has been referenced in 8 publications.

    • Fang S  et al. Arsenic trioxide induces macrophage autophagy and atheroprotection by regulating ROS-dependent TFEB nuclear translocation and AKT/mTOR pathway. Cell Death Dis 12:88 (2021). PubMed: 33462182
    • Xu H  et al. Luteolin Attenuates Doxorubicin-Induced Cardiotoxicity Through Promoting Mitochondrial Autophagy. Front Physiol 11:113 (2020). PubMed: 32116805
    • Xue J  et al. Addition of High Molecular Weight Hyaluronic Acid to Fibroblast-Like Stromal Cells Modulates Endogenous Hyaluronic Acid Metabolism and Enhances Proteolytic Processing and Secretion of Versican. Cells 9:N/A (2020). PubMed: 32668663
    • Chen H  et al. Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production. JCI Insight 5:N/A (2020). PubMed: 32879135
    • Sui B  et al. A novel antiviral lncRNA, EDAL, shields a T309 O-GlcNAcylation site to promote EZH2 lysosomal degradation. Genome Biol 21:228 (2020). PubMed: 32873321
    • Labadie T & Roy P A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion. PLoS Pathog 16:e1009015 (2020). PubMed: 33075107
    • Serramito-Gómez I  et al. Regulation of cytokine signaling through direct interaction between cytokine receptors and the ATG16L1 WD40 domain. Nat Commun 11:5919 (2020). PubMed: 33219218
    • Trubetckaia O  et al. Alpha-synuclein is strategically positioned for afferent modulation of midbrain dopamine neurons and is essential for cocaine preference. Commun Biol 2:418 (2019). PubMed: 31754648

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-LAMP1 antibody [EPR21026]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (OC-2 cell (organ of Corti))
    Permeabilization
    Yes - 0.3% Triton x100
    Specification
    OC-2 cell (organ of Corti)
    Blocking step
    BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

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    Submitted Feb 10 2020

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