Anti-LAMP1 antibody [LY1C6] (ab13523)
Key features and details
- Mouse monoclonal [LY1C6] to LAMP1
- Suitable for: ICC, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-LAMP1 antibody [LY1C6]
See all LAMP1 primary antibodies -
Description
Mouse monoclonal [LY1C6] to LAMP1 -
Host species
Mouse -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Tissue, cells or virus corresponding to Rat LAMP1. Rat liver lysosomal membrane preparations
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Positive control
- Highly purified rat lysosomal membrane preparations.
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General notes
For maximum product recovery centrifuge the product vial before removing cap.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
LY1C6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC | (1) |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa. |
Target
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Function
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 3916 Human
- Entrez Gene: 25328 Rat
- Omim: 153330 Human
- SwissProt: P11279 Human
- SwissProt: P14562 Rat
- Unigene: 494419 Human
- Unigene: 40177 Rat
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Alternative names
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
Images
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Anti-LAMP1 antibody [LY1C6] (ab13523) at 1/1000 dilution + Rat liver microsome lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP -
ab13523 at 1/100 dilution staining LAMP1 in LAMP1 transfected HeLa cells by Immunocytochemistry/ immunofluorescence. A Alexa Fluor® 568 conjugated goat anti mouse was used at 1/50 dilution as secondary.
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ab13523 used in Western Blot. Lysates prepared from CHO cells stably expressing aIIbß3 integrins (A5 cells) were used.
WCL, N/IC, C, P, and PM indicate whole cell lysates, nuclear/intact cell, cytosolic, high-speed pellet, and plasma membrane fractions, respectively.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab13523 has been referenced in 26 publications.
- Gatault P et al. Rat and human cytomegalovirus ORF116 encodes a virion envelope glycoprotein required for infectivity. Virology 557:23-33 (2021). PubMed: 33601113
- Low SW et al. Development and characterization of a monoclonal antibody blocking human TRPM4 channel. Sci Rep 11:10411 (2021). PubMed: 34002002
- Liu X et al. PHLPP Sensitizes Multiple Myeloma Cells to Bortezomib Through Regulating LAMP2. Onco Targets Ther 13:401-411 (2020). PubMed: 32021285
- Huang S et al. Autophagy is involved in the protective effect of p21 on LPS-induced cardiac dysfunction. Cell Death Dis 11:554 (2020). PubMed: 32694519
- Demirev AV et al. V232M substitution restricts a distinct O-glycosylation of PLD3 and its neuroprotective function. Neurobiol Dis 129:182-194 (2019). PubMed: 31121321