Anti-LAMP1 antibody - Lysosome Marker (ab24170)
- Datasheet
- References (297)
- Protocols
Overview
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Product nameAnti-LAMP1 antibody - Lysosome Marker
See all LAMP1 primary antibodies -
DescriptionRabbit polyclonal to LAMP1 - Lysosome Marker
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Host speciesRabbit
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Tested applicationsSuitable for: IP, IHC-P, WB, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Chicken, Hamster, Cat, Dog, Human, Xenopus laevis, Zebrafish, African green monkey
Predicted to work with: Cow -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human LAMP1.
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Positive control
- WB: Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line), HEK293 (Human embryonic kidney cell line) and MCF7 (Human breast adenocarcinoma cell line) whole cell lysates. IHC-P: FFPE human normal kidney tissue sections.
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General notes
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading...
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PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab24170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP | Use at an assay dependent concentration. PubMed: 21152024 | |
IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 90-130 kDa (predicted molecular weight: 120 kDa). Some variability in MW may be observed due to differing levels of glycosylation of the target protein in different cell/tissue types. Abcam recommends using Milk as the blocking agent. |
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IHC-Fr | Use at an assay dependent concentration. |
Target
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FunctionPresents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
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Sequence similaritiesBelongs to the LAMP family.
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Post-translational
modificationsO- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. -
Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
- Information by UniProt
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Database links
- Entrez Gene: 3916 Human
- Entrez Gene: 16783 Mouse
- Entrez Gene: 25328 Rat
- Omim: 153330 Human
- SwissProt: P11279 Human
- SwissProt: P11438 Mouse
- SwissProt: P14562 Rat
- Unigene: 494419 Human
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Alternative names
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
IHC image of LAMP1 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24170, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml
Lane 1 : Jurkat (Human) Whole Cell Lysate
Lane 2 : HEK293 (Human) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab24170 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an Abreview submitted by Mr Carl Hobbsab24170 staining LAMP1 in human kidney tissue sections. Staining correlates with lysosomal specificity, particularly in the proximal convoluted tubules where lysosomes are enriched. Formalin/PFA-fixed human kidney tissue sections were incubated with ab24170 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Please see accompanying abreview for additional information.
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All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Additional bands at: 23 kDa, 35 kDa, 45 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an abreview submitted by Dr. Martin Broadstock (King's College London, United Kingdom)
IHC-P image of LAMP1 staining on human Cortex sections using ab24170 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retreival using citric acid. The sections were then permeabilized using 0.05% Tween-20 and blocking was performed using 3% BSA for 1 hour at 21°C. The primary antibody ab24170 was diluted using 3% BSA with 0.05% Tween-20 in PBS and incubated with the sections for 18 hours at 4°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:500)
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Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an anonymous AbreviewAnti-LAMP1 antibody - Lysosome Marker (ab24170) at 1/700 dilution (in 5% milk for 4 hours at 20°C) + Rat Kidney - whole tissue lysate at 18 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Exposure time: 5 minutes
Blocking Step: 5% Milk for 1 hour at 20°C
Protocols
References
This product has been referenced in:
- Liu H et al. Mitophagy protects SH-SY5Y neuroblastoma cells against the TNFa-induced inflammatory injury: Involvement of microRNA-145 and Bnip3. Biomed Pharmacother 109:957-968 (2019). Read more (PubMed: 30551550) »
- Martínez-Fábregas J et al. Lysosomal protease deficiency or substrate overload induces an oxidative-stress mediated STAT3-dependent pathway of lysosomal homeostasis. Nat Commun 9:5343 (2018). Read more (PubMed: 30559339) »