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    lamp1-antibody-lysosome-marker-ab24170.pdf

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Anti-LAMP1 antibody - Lysosome Marker (ab24170)

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  • SDS
Reviews (89)Q&A (6)References (478)

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Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)

Key features and details

  • Rabbit polyclonal to LAMP1 - Lysosome Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-LAMP1 antibody - Lysosome Marker
    See all LAMP1 primary antibodies
  • Description

    Rabbit polyclonal to LAMP1 - Lysosome Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Hamster, Cow, Cat, Dog, Xenopus laevis, Monkey, Zebrafish, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human LAMP1 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab25744)

  • General notes

      

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Signal Transduction
    • Antibodies
    • lamp1

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human LAMP1 peptide (ab25744)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human LAMP1 protein (ab151863)
  • Related Products

    • Lysosomal Staining Kit - Blue Fluorescence - Cytopainter (ab112135)
    • Lysosomal Staining Kit - Red Fluorescence - Cytopainter (ab112137)
    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab24170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (10)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (34)
Use a concentration of 1 µg/ml. Detects a band of approximately 90-130 kDa (predicted molecular weight: 120 kDa).

Some variability in MW may be observed due to differing levels of glycosylation of the target protein in different cell/tissue types. Abcam recommends using Milk as the blocking agent. 

Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 90-130 kDa (predicted molecular weight: 120 kDa).

Some variability in MW may be observed due to differing levels of glycosylation of the target protein in different cell/tissue types. Abcam recommends using Milk as the blocking agent. 

Target

  • Function

    Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
  • Sequence similarities

    Belongs to the LAMP family.
  • Post-translational
    modifications

    O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
  • Cellular localization

    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Target information above from: UniProt accession P11279 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3916 Human
    • Entrez Gene: 16783 Mouse
    • Entrez Gene: 25328 Rat
    • Omim: 153330 Human
    • SwissProt: P11279 Human
    • SwissProt: P11438 Mouse
    • SwissProt: P14562 Rat
    • Unigene: 494419 Human
    • Unigene: 16716 Mouse
    • Unigene: 475822 Mouse
    • Unigene: 40177 Rat
    see all
  • Alternative names

    • CD107 antigen like family member A antibody
    • CD107 antigen-like family member A antibody
    • CD107a antibody
    • CD107a antigen antibody
    • LAMP 1 antibody
    • LAMP-1 antibody
    • LAMP1 antibody
    • LAMP1_HUMAN antibody
    • LAMPA antibody
    • LGP120 antibody
    • lgpA antibody
    • Lysosomal membrane glycoprotein 120KD antibody
    • Lysosomal Associated Membrane Protein 1 antibody
    • Lysosome associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane glycoprotein 1 antibody
    • Lysosome-associated membrane protein 1 antibody
    • OTTHUMP00000040663 antibody
    see all

Images

  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml

    Lane 1 : Jurkat (Human) Whole Cell Lysate
    Lane 2 : HEK293 (Human) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 120 kDa
    Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab24170 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)

    IHC image of LAMP1 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24170, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)

    Immunohistochemical analysis of dog retina stained for LAMP1 (red) using ab24170 at 1:200, followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Alexa Fluor® 568-conjugated at 1:500. Counterstain: DAPI (blue).

    See Abreview

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)

    Immunohistochemical analysis of cat retina stained for LAMP1 (red) using ab24170 at 1:200, followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Alexa Fluor® 568-conjugated at 1:500. Counterstain: DAPI (blue).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an Abreview submitted by Mr Carl Hobbs
    ab24170 staining LAMP1 in human kidney tissue sections. Staining correlates with lysosomal specificity, particularly in the proximal convoluted tubules where lysosomes are enriched. Formalin/PFA-fixed human kidney tissue sections were incubated with ab24170 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Please see accompanying abreview for additional information.

    See Abreview

  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    All lanes : Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 120 kDa
    Additional bands at: 23 kDa, 35 kDa, 45 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an abreview submitted by Dr. Martin Broadstock (King's College London, United Kingdom)

    IHC-P image of LAMP1 staining on human Cortex sections using ab24170 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retreival using citric acid. The sections were then permeabilized using 0.05% Tween-20 and blocking was performed using 3% BSA for 1 hour at 21°C. The primary antibody ab24170 was diluted using  3% BSA with 0.05% Tween-20 in PBS and incubated  with the sections for 18 hours at 4°C. The secondary antibody used was Goat polyclonal to rabbit IgG conjugated to biotin (1:500)

    See Abreview

  • Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)
    Western blot - Anti-LAMP1 antibody - Lysosome Marker (ab24170)This image is courtesy of an anonymous Abreview
    Anti-LAMP1 antibody - Lysosome Marker (ab24170) at 1/700 dilution (in 5% milk for 4 hours at 20°C) + Rat Kidney - whole tissue lysate at 18 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 120 kDa
    Observed band size: 120 kDa


    Exposure time: 5 minutes


    Blocking Step: 5% Milk for 1 hour at 20°C

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (478)

Publishing research using ab24170? Please let us know so that we can cite the reference in this datasheet.

ab24170 has been referenced in 478 publications.

  • Rostami J  et al. Astrocytes have the capacity to act as antigen-presenting cells in the Parkinson's disease brain. J Neuroinflammation 17:119 (2020). PubMed: 32299492
  • Duan C  et al. Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH- pathways. Cell Death Dis 11:251 (2020). PubMed: 32312970
  • Wei J  et al. TRPV1 activation mitigates hypoxic injury in mouse cardiomyocytes by inducing autophagy through the AMPK signaling pathway. Am J Physiol Cell Physiol 318:C1018-C1029 (2020). PubMed: 32293932
  • Hintze S  et al. Moss-Derived Human Recombinant GAA Provides an Optimized Enzyme Uptake in Differentiated Human Muscle Cells of Pompe Disease. Int J Mol Sci 21:N/A (2020). PubMed: 32290314
  • Lisjak M  et al. Indirect Role of AQP4b and AQP4d Isoforms in Dynamics of Astrocyte Volume and Orthogonal Arrays of Particles. Cells 9:N/A (2020). PubMed: 32192013
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 89 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-LAMP1 antibody - Lysosome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Vero cells)
Permeabilization
Yes - Saponin
Specification
Vero cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 25 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LAMP1 antibody - Lysosome Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization
Yes - PBST(0.5% v/v triton X-100, sigma)
Specification
tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 16 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-LAMP1 antibody - Lysosome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Dog Tissue sections (Retina)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% TritonX-100 + 0.05% Tween20
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 27 2020

Western blot abreview for Anti-LAMP1 antibody - Lysosome Marker

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50000 cells
Specification
Glioblastoma
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Aug 15 2019

Western blot abreview for Anti-LAMP1 antibody - Lysosome Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jul 22 2019

Western blot abreview for Anti-LAMP1 antibody - Lysosome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Gerbil Cell lysate - whole cell (Mongolian gerbil brain)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Mongolian gerbil brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted May 27 2019

Immunohistochemistry free floating abreview for Anti-LAMP1 antibody - Lysosome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (PFA fixed Brain)
Specification
PFA fixed Brain
Read More

Abcam user community

Verified customer

Submitted Feb 14 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-LAMP1 antibody - Lysosome Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.3% triton x100
Specification
HeLa
Blocking step
BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 09 2018

IHC - Wholemount abreview for Anti-LAMP1 antibody - Lysosome Marker

Good
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (intestine organoids)
Specification
intestine organoids
Read More

Abcam user community

Verified customer

Submitted Jul 11 2018

Immunohistochemistry (Frozen sections) abreview for Anti-LAMP1 antibody - Lysosome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (tumor)
Permeabilization
No
Specification
tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 30 2018

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