Key features and details
- Rabbit polyclonal to LAMP2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-LAMP2 antibody
See all LAMP2 primary antibodies
DescriptionRabbit polyclonal to LAMP2
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
- IHC-P: Mouse lung tissue. WB: Mouse intestine, liver and placenta lysates. Flow Cyt: U937 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol
Aqueous buffered solution.
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab203224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 45 kDa.|
|IHC-P||1/100 - 1/500.
(or 1/50 – 1/200 using a fluorescent secondary antibody).
|Flow Cyt||1/20 - 1/100.|
FunctionImplicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
Tissue specificityIsoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
Involvement in diseaseDefects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
Sequence similaritiesBelongs to the LAMP family.
modificationsO- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
- Information by UniProt
FormAlternative splicing produces 3 isoforms.
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
- CD107b antibody
All lanes : Anti-LAMP2 antibody (ab203224) at 1/200 dilution
Lane 1 : Mouse intestine lysate
Lane 2 : Mouse liver lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 45 kDa
Flow cytometric analysis of U937 (human histiocytic lymphoma cell line) cells labeling LAMP2 withab203224 at 1/100 dilution for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes, compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Anti-LAMP2 antibody (ab203224) at 1/100 dilution + Mouse placenta lysate
Predicted band size: 45 kDa
Immunohistochemical analysis of formalin-fixed, paraffin embedded mouse lung tissue labelling LAMP2 with ab203224 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab203224 has been referenced in 3 publications.
- Chen H et al. Effect of autophagy on allodynia, hyperalgesia and astrocyte activation in a rat model of neuropathic pain. Int J Mol Med 42:2009-2019 (2018). PubMed: 30015858
- Zhang LX et al. Niclosamide attenuates inflammatory cytokines via the autophagy pathway leading to improved outcomes in renal ischemia/reperfusion injury. Mol Med Rep 16:1810-1816 (2017). PubMed: 28627643
- Murgai M et al. KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis. Nat Med 23:1176-1190 (2017). PubMed: 28920957