Product nameAnti-LAMP2 antibody [ABL-93]
See all LAMP2 primary antibodies
DescriptionRat monoclonal [ABL-93] to LAMP2
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IP, ICC/IF, WBmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse LAMP2.
- ICC/IF: RAW246.7 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 8.20
Constituent: 100% Borate buffered saline
Concentration information loading...
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab25339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IF||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.|
FunctionImplicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
Tissue specificityIsoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
Involvement in diseaseDefects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
Sequence similaritiesBelongs to the LAMP family.
modificationsO- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
- Information by UniProt
FormAlternative splicing produces 3 isoforms.
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
- CD107b antibody
Immunofluorescence analysis of mouse A20 B cells, staining LAMP2 with ab25339.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 5% FCS in PBS) for 24 hours at 4°C. A FITC-conjugated goat anti-rat polyclonal IgG (1/500) was used as the secondary antibody.
ab25339 staining LAMP2 from mouse liver cells by Immunohistochemistry (Frozen sections). The fresh tissue was cut into small pieces, put into OCT, and frozen in liquid nitrogen. 5µm sections were prepared using a cyrostat. The tissue section was fixed in 5% paraformaldehyde and blocked in 5% serum with 0.05% saponin to increase the permeability. The sample was incubated with ab25339 (1/100 dilution) overnight, and incubated with secondary antibody (Cy2 conjugated donkey anti-rat IgG) for 2 hours. After staining with DAPI (1/1000 dilution) for 2 minutess, the section was mounted.
All lanes : Anti-LAMP2 antibody [ABL-93] (ab25339) at 1/1000 dilution
Lane 1 : MEF1 lysate
Lane 2 : RAW264.7 lysate
Lysates/proteins at 20 µg per lane.
All lanes : Peroxidase labelled goat anti-rat IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Additional bands at: 120 kDa (possible post-translational modification), 90 kDa (possible post-translational modification)
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system under denaturing, reducing conditions. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. After transfer, the membrane was blocked for an hour in 2% BSA before being incubated overnight at 4°C with a rat monoclonal [ABL-93] to LAMP2 (ab25339; diluted 1:1000). Antibody binding was detected using peroxidase labelled goat anti-rat IgG (diluted 1:10000) for an hour at room temperature and visualised using ECL development solution (20 minutes exposure).
Overlay histogram showing RAW 264.7 cells stained with ab25339 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25339, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
ICC/IF image of ab25339 stained RAW246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25339, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96887, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Chen D et al. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype. Nat Commun 9:873 (2018). Read more (PubMed: 29491374) »
- Brosius Lutz A et al. Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury. Proc Natl Acad Sci U S A 114:E8072-E8080 (2017). IHC . Read more (PubMed: 28874532) »