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    lamp2-antibody-epr21214-ab213294.pdf

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Tags & Cell Markers Subcellular Markers Organelles Lysosome
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Recombinant

Recombinant Anti-LAMP2 antibody [EPR21214] (ab213294)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR21214] (ab213294)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR21214] (ab213294)
  • Anti-LAMP2 antibody [EPR21214] (ab213294)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [EPR21214] to LAMP2
  • Suitable for: IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-LAMP2 antibody [EPR21214]
    See all LAMP2 primary antibodies
  • Description

    Human monoclonal [EPR21214] to LAMP2
  • Host species

    Human
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: FFPE human and mouse normal liver sections.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody.  
    Human monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR21214
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Autophagy
    • Autophagosome
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Myeloid Progenitor
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Autophagosome
    • Cancer
    • Cell Death
    • Autophagy
    • Autophagosome
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 488) (ab200622)
    • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Alexa Fluor® 647) (ab200623)
    • Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (Biotin) (ab201485)
    • Rabbit Anti-Human IgG H&L (HRP) (ab6759)
  • Isotype control

    • Human IgG1, kappa - Isotype Control (ab206198)
  • Recombinant Protein

    • Recombinant Human LAMP2 protein (ab114167)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
Notes
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Function

    Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
  • Tissue specificity

    Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
  • Involvement in disease

    Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
  • Sequence similarities

    Belongs to the LAMP family.
  • Post-translational
    modifications

    O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
  • Cellular localization

    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Target information above from: UniProt accession P13473 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3920 Human
    • Entrez Gene: 16784 Mouse
    • Omim: 309060 Human
    • SwissProt: P13473 Human
    • SwissProt: P17047 Mouse
    • Unigene: 496684 Human
    • Unigene: 486 Mouse
    • Form

      Alternative splicing produces 3 isoforms.
    • Alternative names

      • CD107 antigen like family member B antibody
      • CD107 antigen-like family member B antibody
      • CD107b antibody
      • LAMP 2 antibody
      • LAMP 2C antibody
      • LAMP-2 antibody
      • LAMP2 antibody
      • LAMP2_HUMAN antibody
      • LAMPB antibody
      • LGP 96 antibody
      • LGP110 antibody
      • LGP96 antibody
      • Lysosomal associated membrane protein 2 antibody
      • Lysosome associated membrane glycoprotein 2 antibody
      • Lysosome associated membrane protein 2 antibody
      • Lysosome-associated membrane glycoprotein 2 antibody
      • Lysosome-associated membrane protein 2 antibody
      • MAC3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR21214] (ab213294)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR21214] (ab213294)

      IHC image of LAMP2 staining in a section of formalin-fixed paraffin-embedded normal human liver* performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213294, 5ug/ml, for 15 mins at room temperature. An HRP-conjugated goat anti-Human IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR21214] (ab213294)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR21214] (ab213294)

      IHC image of LAMP2 staining in a section of formalin-fixed paraffin-embedded normal mouse liver performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213294, 5ug/ml, for 15 mins at room temperature. An HRP-conjugated goat anti-Human IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    • Anti-LAMP2 antibody [EPR21214] (ab213294)
      Anti-LAMP2 antibody [EPR21214] (ab213294)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab213294? Please let us know so that we can cite the reference in this datasheet.

    ab213294 has been referenced in 1 publication.

    • Ho PW  et al. Age-dependent accumulation of oligomeric SNCA/a-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA). Autophagy 16:347-370 (2020). PubMed: 30983487

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