Overview

  • Product name
    Anti-LAMP2 antibody [GL2A7]
    See all LAMP2 primary antibodies
  • Description
    Rat monoclonal [GL2A7] to LAMP2
  • Host species
    Rat
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Fr, IP, ICC, ICC/IF, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit
  • Immunogen

    Tissue, cells or virus corresponding to Mouse LAMP2.

  • Positive control
    • WB: HEK-293 cell lysate. ICC/IF: Adult mouse cardiac myocytes; rabbit corneal endothelial cells. IHC-Fr: Mouse uterus tissue.
  • General notes
    For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Properties

Applications

Our Abpromise guarantee covers the use of ab13524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration. (PMID 2142158).
ICC 1/100. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.
ICC/IF 1/100. (see Abreview)
WB Use at an assay dependent concentration. See Abreview.
IHC-FoFr 1/100. (PubMed:17425404)

Target

  • Function
    Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
  • Tissue specificity
    Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
  • Involvement in disease
    Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
  • Cellular localization
    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Information by UniProt
  • Database links
  • Form
    Alternative splicing produces 3 isoforms.
  • Alternative names
    • CD107 antigen like family member B antibody
    • CD107 antigen-like family member B antibody
    • CD107b antibody
    • LAMP 2 antibody
    • LAMP 2C antibody
    • LAMP-2 antibody
    • LAMP2 antibody
    • LAMP2_HUMAN antibody
    • LAMPB antibody
    • LGP 96 antibody
    • LGP110 antibody
    • LGP96 antibody
    • Lysosomal associated membrane protein 2 antibody
    • Lysosome associated membrane glycoprotein 2 antibody
    • Lysosome associated membrane protein 2 antibody
    • Lysosome-associated membrane glycoprotein 2 antibody
    • Lysosome-associated membrane protein 2 antibody
    • MAC3 antibody
    see all

Images

  • Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg

    Secondary
    Goat anti-rat HRP at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 105 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)


    Exposure time: 3 minutes


    Blocked with 5% milk for 1 hour at RT.

    Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.

    See Abreview

  • Immunofluorescence staining of LAMP2 in adult mouse cardiac myocytes using ab13524 at 1/100. Top panels: WT. Lower panels: PINK -/- myocytes. Cells were fixed in 4% PFA and permeabilized with 0.2% saponin. Blocking was performed with 5% normal goat serum + 0.2% Triton X100 in PBS. Hoechst 33342 was used for counter-staining.

  • PFA perfusion-fixed, frozen section of mouse brain tissue stained for LAMP2 using ab13524 at 1/500 dilution in immunohistochemical analysis. Goat anti-rat AlexaFluor® 488 secondary antibody.

    See Abreview

  • ab13524 staining LAMP2 in Mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Rabbit corneal endothelial cell stained for LAMP2 (green) using ab13524 at 1/1000 dilution in ICC/IF.

References

This product has been referenced in:
See all 65 Publications for this product

Customer reviews and Q&As

1-10 of 20 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 18 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 18 2018

Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 16 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% saponin
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 27 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 10% gel)
Loading amount
30 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Insu Kwon

Verified customer

Submitted Dec 10 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC IN A PT LINK
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 24 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Aplysia Tissue sections (ganglia)
Specification
ganglia
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted Dec 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Uterus)
Permeabilization
No
Specification
Uterus
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
4 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Mouse Tissue lysate - whole (uterus)
Specification
uterus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Varkha Agrawal

Verified customer

Submitted Sep 24 2013

Answer

Thank you for your enquiry.

I have obtained some more details regarding these antibodies which I hope will be helpful:

ab13524 Anti-LAMP2 antibody [GL2A7] The immunogen used to generate this antibody was purified mouse liver lysosomal membranes. This binds specifically to presumptive lysosomes and late endosomes. However the exact antigen that the antibody binds has not been sequenced so I am sorry information regarding which isoforms this detects and which epitope is regrettably not available.

ab37024 is raised against C-terminal region of isoform B. The immunogen sequence has 35% alignment with isoform A and 64% with isoform C: It is therefore not likely to detect isoform A and C.

I hope this information is helpful and helps to explain the results. If you have any further concerns regarding the results, please do not hesitate to contact me again with further details and I will be happy to investigate this for you.

Read More

1-10 of 20 Abreviews or Q&A

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