Anti-LAMP2 antibody [GL2A7] (ab13524)
- Datasheet
- References (72)
- Protocols
Overview
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Product nameAnti-LAMP2 antibody [GL2A7]
See all LAMP2 primary antibodies -
DescriptionRat monoclonal [GL2A7] to LAMP2
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Host speciesRat
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Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, IP, ICC, ICC/IF, WB, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Rabbit
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Immunogen
Tissue, cells or virus corresponding to Mouse LAMP2.
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Positive control
- WB: HEK-293 cell lysate. ICC/IF: Adult mouse cardiac myocytes; rabbit corneal endothelial cells. IHC-Fr: Mouse uterus tissue.
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General notesFor maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberGL2A7
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab13524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. (PMID 2142158). | |
| ICC | 1/100. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. | |
| ICC/IF | 1/100. (see Abreview) | |
| WB | Use at an assay dependent concentration. See Abreview. | |
| IHC-FoFr | 1/100. (PubMed:17425404) |
Target
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FunctionImplicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
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Tissue specificityIsoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
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Involvement in diseaseDefects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
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Sequence similaritiesBelongs to the LAMP family.
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Post-translational
modificationsO- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. -
Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
- Information by UniProt
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Database links
- Entrez Gene: 16784 Mouse
- SwissProt: P17047 Mouse
- Unigene: 486 Mouse
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FormAlternative splicing produces 3 isoforms.
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Alternative names
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
- CD107b antibody
see all
Images
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![Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.com/ps/products/13/ab13524/Images/ab13524-260757-13524-WB.jpg)
Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)This image is courtesy of an anonymous Abreview.Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg
Secondary
Goat anti-rat HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)
Exposure time: 3 minutesBlocked with 5% milk for 1 hour at RT.
Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.
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![Immunocytochemistry/ Immunofluorescence - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.com/ps/products/13/ab13524/Images/ab13524-260759-13524ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LAMP2 antibody [GL2A7] (ab13524)Image from Kubli DA et al., Plos One. 2015;10(6):e0130707. Fig 5.; doi: 10.1371/journal.pone.0130707Immunofluorescence staining of LAMP2 in adult mouse cardiac myocytes using ab13524 at 1/100. Top panels: WT. Lower panels: PINK -/- myocytes. Cells were fixed in 4% PFA and permeabilized with 0.2% saponin. Blocking was performed with 5% normal goat serum + 0.2% Triton X100 in PBS. Hoechst 33342 was used for counter-staining.
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![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.com/ps/products/13/ab13524/Images/ab13524-308660-24-q.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-LAMP2 antibody [GL2A7] (ab13524)Image courtesy of an anonymous AbReviewPFA perfusion-fixed, frozen section of mouse brain tissue stained for LAMP2 using ab13524 at 1/500 dilution in immunohistochemical analysis. Goat anti-rat AlexaFluor® 488 secondary antibody.
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![Immunohistochemistry (Frozen sections) - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.com/ps/products/13/ab13524/Images/ab13524-212781-ab13524ihcf.jpg)
Immunohistochemistry (Frozen sections) - Anti-LAMP2 antibody [GL2A7] (ab13524)This image is courtesy of an Abreview submitted by Mukesh Jaiswalab13524 staining LAMP2 in Mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.com/ps/products/13/ab13524/Images/ab13524-308659-24j.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LAMP2 antibody [GL2A7] (ab13524)Rabbit corneal endothelial cell stained for LAMP2 (green) using ab13524 at 1/1000 dilution in ICC/IF.
Protocols
References
This product has been referenced in:
- Lloyd-Lewis B et al. Stat3-mediated alterations in lysosomal membrane protein composition. J Biol Chem 293:4244-4261 (2018). Read more (PubMed: 29343516) »
- Chen J et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing. Nature N/A:N/A (2018). Read more (PubMed: 29769719) »
