Anti-LAMP2 antibody [GL2A7] (ab13524)
![Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.com/ps/products/13/ab13524/Images/ab13524-397579-AntiLAMP2-antibody-GL2A7-western-blot-nih3t3-hek293-human-mouse.jpg "Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)")
Key features and details
- Rat monoclonal [GL2A7] to LAMP2
- Suitable for: IHC-Fr, ICC, ICC/IF, IHC-FoFr, IP, Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rabbit, Human
- Isotype: IgG
Overview
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Product name
Anti-LAMP2 antibody [GL2A7]
See all LAMP2 primary antibodies -
Description
Rat monoclonal [GL2A7] to LAMP2 -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, ICC, ICC/IF, IHC-FoFr, IP, Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rabbit, Human -
Immunogen
Tissue, cells or virus corresponding to Mouse LAMP2. Purified preparation of mouse liver lysosomal membranes.
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Positive control
- WB: HEK-293 and NIH/3T3 cell lysate.
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General notes
For maximum product recovery, after thawing, centrifuge the product vial before removing cap.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
GL2A7 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| ICC |
Use at an assay dependent concentration.
Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. |
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
See Abreview. |
| IHC-FoFr | (1) |
Use at an assay dependent concentration.
(PubMed:17425404) |
| IP |
Use at an assay dependent concentration.
(PMID 2142158). |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (7) |
1/500.
|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. |
|
ICC/IF
Use at an assay dependent concentration. See Abreview. |
|
IHC-FoFr
Use at an assay dependent concentration. (PubMed:17425404) |
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IP
Use at an assay dependent concentration. (PMID 2142158). |
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Flow Cyt
Use at an assay dependent concentration. ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/500. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. -
Tissue specificity
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver. -
Involvement in disease
Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 3920 Human
- Entrez Gene: 16784 Mouse
- Omim: 309060 Human
- SwissProt: P13473 Human
- SwissProt: P17047 Mouse
- Unigene: 496684 Human
- Unigene: 486 Mouse
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Form
Alternative splicing produces 3 isoforms. -
Alternative names
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
- CD107b antibody
see all
Images
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Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)All lanes : Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/500 dilution (1 hr at RT)
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate at 10 µg
Secondary
All lanes : HRP Goat Anti-Rat, 1 hour at RT at 1/100 dilutionBlock: 5% milk + TBST for 1 hour at RT.
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![Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)](https://www.abcam.com/ps/products/13/ab13524/Images/ab13524-260757-anti-lamp2-antibody-gl2a7-western-blot.jpg)
Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)This image is courtesy of an anonymous Abreview.Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg
Secondary
Goat anti-rat HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)
Exposure time: 3 minutesBlocked with 5% milk for 1 hour at RT.
Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (149)
ab13524 has been referenced in 149 publications.
- Kwon I et al. Endurance Exercise-Induced Autophagy/Mitophagy Coincides with a Reinforced Anabolic State and Increased Mitochondrial Turnover in the Cortex of Young Male Mouse Brain. J Mol Neurosci 71:42-54 (2021). PubMed: 32535714
- Ito J et al. Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice. Elife 10:N/A (2021). PubMed: 33526170
- Yagi M et al. Mitochondrial translation deficiency impairs NAD+ -mediated lysosomal acidification. EMBO J 40:e105268 (2021). PubMed: 33528041
- Fu R et al. Rab27a Contributes to Cathepsin S Secretion in Lacrimal Gland Acinar Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33562815
- Li Y et al. Plin2 deletion increases cholesteryl ester lipid droplet content and disturbs cholesterol balance in adrenal cortex. J Lipid Res 62:100048 (2021). PubMed: 33582145