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  1. Link

    lamp2-antibody-gl2a7-ab13524.pdf

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Tags & Cell Markers Subcellular Markers Organelles Lysosome
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Anti-LAMP2 antibody [GL2A7] (ab13524)

  • Datasheet
  • SDS
Reviews (13)Q&A (8)References (151)

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Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)
  • Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)

Key features and details

  • Rat monoclonal [GL2A7] to LAMP2
  • Suitable for: IHC-Fr, ICC, ICC/IF, IHC-FoFr, IP, Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rabbit, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-LAMP2 antibody [GL2A7]
    See all LAMP2 primary antibodies
  • Description

    Rat monoclonal [GL2A7] to LAMP2
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-Fr, ICC, ICC/IF, IHC-FoFr, IP, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rabbit, Human
  • Immunogen

    Tissue, cells or virus corresponding to Mouse LAMP2. Purified preparation of mouse liver lysosomal membranes.

  • Positive control

    • WB: HEK-293 and NIH/3T3 cell lysate.
  • General notes

    For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    GL2A7
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Cardiovascular
    • Heart
    • Autophagy
    • Autophagosome
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Myeloid Progenitor
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Autophagosome
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Autophagy
    • Autophagosome
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG - Isotype Control (ab37361)
  • Recombinant Protein

    • Recombinant Human LAMP2 protein (ab114167)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab13524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (2)
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.

Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.

ICC/IF (2)
Use at an assay dependent concentration.

See Abreview.

IHC-FoFr (1)
Use at an assay dependent concentration.

(PubMed:17425404)

IP
Use at an assay dependent concentration.

(PMID 2142158).

Flow Cyt
Use at an assay dependent concentration.

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB (7)
1/500.
IHC-P (1)
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.

Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.

ICC/IF
Use at an assay dependent concentration.

See Abreview.

IHC-FoFr
Use at an assay dependent concentration.

(PubMed:17425404)

IP
Use at an assay dependent concentration.

(PMID 2142158).

Flow Cyt
Use at an assay dependent concentration.

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/500.
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
  • Tissue specificity

    Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
  • Involvement in disease

    Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
  • Sequence similarities

    Belongs to the LAMP family.
  • Post-translational
    modifications

    O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
  • Cellular localization

    Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
  • Target information above from: UniProt accession P13473 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3920 Human
    • Entrez Gene: 16784 Mouse
    • Omim: 309060 Human
    • SwissProt: P13473 Human
    • SwissProt: P17047 Mouse
    • Unigene: 496684 Human
    • Unigene: 486 Mouse
    • Form

      Alternative splicing produces 3 isoforms.
    • Alternative names

      • CD107 antigen like family member B antibody
      • CD107 antigen-like family member B antibody
      • CD107b antibody
      • LAMP 2 antibody
      • LAMP 2C antibody
      • LAMP-2 antibody
      • LAMP2 antibody
      • LAMP2_HUMAN antibody
      • LAMPB antibody
      • LGP 96 antibody
      • LGP110 antibody
      • LGP96 antibody
      • Lysosomal associated membrane protein 2 antibody
      • Lysosome associated membrane glycoprotein 2 antibody
      • Lysosome associated membrane protein 2 antibody
      • Lysosome-associated membrane glycoprotein 2 antibody
      • Lysosome-associated membrane protein 2 antibody
      • MAC3 antibody
      see all

    Images

    • Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)
      Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)
      All lanes : Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/500 dilution (1 hr at RT)

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg
      Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate at 10 µg

      Secondary
      All lanes : HRP Goat Anti-Rat, 1 hour at RT at 1/100 dilution


      Block: 5% milk + TBST for 1 hour at RT.

    • Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)
      Western blot - Anti-LAMP2 antibody [GL2A7] (ab13524)This image is courtesy of an anonymous Abreview.
      Anti-LAMP2 antibody [GL2A7] (ab13524) at 1/1000 dilution + HEK293 cell lysate at 20 µg

      Secondary
      Goat anti-rat HRP at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 105 kDa why is the actual band size different from the predicted?
      Additional bands at: 28 kDa (possible non-specific binding), 70 kDa (possible non-specific binding)


      Exposure time: 3 minutes


      Blocked with 5% milk for 1 hour at RT.

      Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.

      See Abreview

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (151)

    Publishing research using ab13524? Please let us know so that we can cite the reference in this datasheet.

    ab13524 has been referenced in 151 publications.

    • Kwon I  et al. Endurance Exercise-Induced Autophagy/Mitophagy Coincides with a Reinforced Anabolic State and Increased Mitochondrial Turnover in the Cortex of Young Male Mouse Brain. J Mol Neurosci 71:42-54 (2021). PubMed: 32535714
    • Ito J  et al. Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice. Elife 10:N/A (2021). PubMed: 33526170
    • Yagi M  et al. Mitochondrial translation deficiency impairs NAD+ -mediated lysosomal acidification. EMBO J 40:e105268 (2021). PubMed: 33528041
    • Fu R  et al. Rab27a Contributes to Cathepsin S Secretion in Lacrimal Gland Acinar Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33562815
    • Li Y  et al. Plin2 deletion increases cholesteryl ester lipid droplet content and disturbs cholesterol balance in adrenal cortex. J Lipid Res 62:100048 (2021). PubMed: 33582145
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    1-10 of 13 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LAMP2 antibody [GL2A7]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (184A1 mammary epithelial)
    Permeabilization
    Yes - PBS + 0.25% Triton-X
    Specification
    184A1 mammary epithelial
    Blocking step
    (agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 17 2020

    Western blot abreview for Anti-LAMP2 antibody [GL2A7]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 18 2018

    Western blot abreview for Anti-LAMP2 antibody [GL2A7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (4-12% gel)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 18 2018

    Western blot abreview for Anti-LAMP2 antibody [GL2A7]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African green monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 16 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-LAMP2 antibody [GL2A7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1% saponin
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 27 2016

    Western blot abreview for Anti-LAMP2 antibody [GL2A7]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (NuPAGE® Novex® 10% gel)
    Loading amount
    30 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    DR. Insu Kwon

    Verified customer

    Submitted Dec 10 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LAMP2 antibody [GL2A7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC IN A PT LINK
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 24 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-LAMP2 antibody [GL2A7]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Aplysia Tissue sections (ganglia)
    Specification
    ganglia
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    MRS. Francoise Geffroy

    Verified customer

    Submitted Dec 09 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-LAMP2 antibody [GL2A7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Uterus)
    Permeabilization
    No
    Specification
    Uterus
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Nov 29 2013

    Western blot abreview for Anti-LAMP2 antibody [GL2A7] - Lysosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    4 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Mouse Tissue lysate - whole (uterus)
    Specification
    uterus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Varkha Agrawal

    Verified customer

    Submitted Sep 24 2013

    1-10 of 13 Abreviews

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