Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab83659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/312500.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 44, 64 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionMight change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells.
Tissue specificityExpressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.
Sequence similaritiesBelongs to the LAMP family.
Cellular localizationLysosome membrane.
- Information by UniProt
- CD208 antibody
- CD208 antigen antibody
- DC LAMP antibody
Anti-LAMP3 antibody (ab83659) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Observed band size: 44,64 kDa why is the actual band size different from the predicted?
Gel concentration 12%
This product has been referenced in:
- Wegmann S et al. Experimental evidence for the age dependence of tau protein spread in the brain. Sci Adv 5:eaaw6404 (2019). Read more (PubMed: 31249873) »
- Liu S et al. LAMP3 plays an oncogenic role in osteosarcoma cells partially by inhibiting TP53. Cell Mol Biol Lett 23:33 (2018). Read more (PubMed: 30008754) »