Anti-LANCL1 antibody (ab229257)
Key features and details
- Rabbit polyclonal to LANCL1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LANCL1 antibody
See all LANCL1 primary antibodies -
Description
Rabbit polyclonal to LANCL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey -
Immunogen
Recombinant fragment within Human LANCL1 (internal sequence). The exact sequence is proprietary.
Database link: O43813 -
Positive control
- IHC-P: U87 xenograft tissue. WB: Jurkat and Raji whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
Target
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Function
May play a role in EPS8 signaling. Binds glutathion. -
Tissue specificity
Detected in erythrocytes, brain, kidney, testis, ovary, heart, lung, placenta and spleen (at protein level). Ubiquitous. Strongly expressed in brain, spinal cord, pituitary gland, kidney, heart, skeletal muscle, pancreas, ovary and testis. -
Sequence similarities
Belongs to the LanC-like protein family. -
Cellular localization
Cytoplasm. Membrane. Nucleus. Detected at the surface of Maurer's clefts in malaria-infected erythrocytes at late stages of parasite development. - Information by UniProt
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Database links
- Entrez Gene: 10314 Human
- Entrez Gene: 14768 Mouse
- Entrez Gene: 114515 Rat
- Entrez Gene: 711723 Rhesus monkey
- Omim: 604155 Human
- SwissProt: O43813 Human
- SwissProt: O89112 Mouse
- SwissProt: Q9QX69 Rat
see all -
Alternative names
- 40 kDa erythrocyte membrane protein antibody
- G protein-coupled receptor 69A antibody
- GPR69A antibody
see all
Images
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Paraffin-embedded U87 xenograft tissue stained for LANCL1 with ab229257 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-LANCL1 antibody (ab229257) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 45 kDa10% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229257 has not yet been referenced specifically in any publications.