Key features and details
- Rabbit polyclonal to LANCL2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LANCL2 antibody
See all LANCL2 primary antibodies
DescriptionRabbit polyclonal to LANCL2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: A549 and U-87 MG whole cell lysate. IHC-P: Human brain tissue. ICC/IF: HepG2 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab237520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 51 kDa.|
|IHC-P||1/200 - 1/500.|
|ICC/IF||1/50 - 1/200.|
FunctionNecessary for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes.
Tissue specificityExpressed in brain and testis.
Sequence similaritiesBelongs to the LanC-like protein family.
modificationsMyristoylated. Essential for membrane association.
Cellular localizationNucleus. Cytoplasm. Cell membrane. Localizes to the juxta-nuclear vesicles. Associates with the cortical actin cytoskeleton. Cholesterol depletion by methyl-beta-cyclodextrin causes partial dissociation from the cell membrane in vitro and an enhanced cell detachment from the matrix in vivo. Membrane-association is important for the increased cellular sensitivity to an anticancer drug.
- Information by UniProt
- G protein coupled receptor 69B antibody
- GPR69B antibody
- LanC (bacterial lantibiotic synthetase component C) like 2 antibody
All lanes : Anti-LANCL2 antibody (ab237520) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Paraffin-embedded human brain tissue stained for LANCL2 using ab237520 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for LANCL2 (Green) using ab237520 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237520 has not yet been referenced specifically in any publications.