Anti-LANCL2 antibody (ab88860)
Key features and details
- Mouse polyclonal to LANCL2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LANCL2 antibody
See all LANCL2 primary antibodies -
Description
Mouse polyclonal to LANCL2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human LANCL2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: A431 cell lysate; LANCL2 transfected 293T cell lysate ICC/IF: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Necessary for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes. -
Tissue specificity
Expressed in brain and testis. -
Sequence similarities
Belongs to the LanC-like protein family. -
Post-translational
modificationsMyristoylated. Essential for membrane association. -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. Localizes to the juxta-nuclear vesicles. Associates with the cortical actin cytoskeleton. Cholesterol depletion by methyl-beta-cyclodextrin causes partial dissociation from the cell membrane in vitro and an enhanced cell detachment from the matrix in vivo. Membrane-association is important for the increased cellular sensitivity to an anticancer drug. - Information by UniProt
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Database links
- Entrez Gene: 55915 Human
- SwissProt: Q9NS86 Human
- Unigene: 595384 Human
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Alternative names
- G protein coupled receptor 69B antibody
- GPR69B antibody
- LanC (bacterial lantibiotic synthetase component C) like 2 antibody
see all
Images
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Anti-LANCL2 antibody (ab88860) at 1 µg/ml + A431 cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: ~48 kDa why is the actual band size different from the predicted? -
All lanes : Anti-LANCL2 antibody (ab88860) at 1 µg/ml
Lane 1 : LANCL2 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 51 kDa
Observed band size: ~45 kDa why is the actual band size different from the predicted? -
ab88860, at 10 µg/ml, staining LANCL2 in paraformaldehyde fixed, permeabilized HeLa cell by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab88860 has been referenced in 1 publication.
- Lou Y et al. Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis. Cell Death Dis 12:170 (2021). PubMed: 33568630