Overview

  • Product name

  • Description

    Rabbit polyclonal to LANCL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 287-336 (EMVKPSIDYV RHKKFRSGNY PSSLSNETDR LVHWCHGAPG VIHMLMQAYK) of Human LANCL2 (NP_061167).

  • Positive control

    • Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Necessary for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes.
  • Tissue specificity

    Expressed in brain and testis.
  • Sequence similarities

    Belongs to the LanC-like protein family.
  • Post-translational
    modifications

    Myristoylated. Essential for membrane association.
  • Cellular localization

    Nucleus. Cytoplasm. Cell membrane. Localizes to the juxta-nuclear vesicles. Associates with the cortical actin cytoskeleton. Cholesterol depletion by methyl-beta-cyclodextrin causes partial dissociation from the cell membrane in vitro and an enhanced cell detachment from the matrix in vivo. Membrane-association is important for the increased cellular sensitivity to an anticancer drug.
  • Information by UniProt
  • Database links

  • Alternative names

    • G protein coupled receptor 69B antibody
    • GPR69B antibody
    • LanC (bacterial lantibiotic synthetase component C) like 2 antibody
    • LanC-like protein 2 antibody
    • LANC2_HUMAN antibody
    • LANCL2 antibody
    • TASP antibody
    • Testis specific adriamycin sensitivity protein antibody
    • Testis-specific adriamycin sensitivity protein antibody
    see all

Images

  • Anti-LANCL2 antibody (ab99083) at 1 µg/ml + Human fetal muscle lysate at 10 µg

    Predicted band size: 51 kDa



    Gel concentration: 12%

References

ab99083 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for taking time to complete our questionnaire and providing this extra data. I am sorry to hear this antibody is not providing satisfactory results. I appreciate the time your customer has spent in the laboratory and understand their concerns. It is regrettable the results have not been successful. Having reviewed the protocol details, and performed the advised protocol tips I believe this product should have given satisfactory results. It appears that you may have received a faulty vial. I apologise for the inconvenience caused by this. I am amle to offer a free of charge replacment with a different batch of this antibody, an alternative antibody (such as ab88860 or ab67676 both mouse polyclonals) or althernatively if this is not satisfactory credit note can be issued in compensation. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Question

DESCRIPTION OF THE PROBLEM Multiple bands SAMPLE •Species: human •What’s cell line or tissue: H1299 and HCC827 •Cell extract or Nuclear extract: cell extract PRIMARY ANTIBODY •Species: rabbit •Reacts against: human •At what dilution(s) have you tested this antibody: 1ug/mL •What dilution buffer was used: 3% BSA •Incubation time: O/N •Incubation temperature: 4℃ •What washing steps were done: 5min x 3 repeats, 10min x 2 repeats DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED Yes, I stripped the membrane, and then hybridized with antibody against v5 tag. ( I transfected v5-taged plasmid to the cells) ANTIBODY STORAGE CONDITIONS -20℃ SAMPLE PREPARATION •What lysis buffer was used: M-Per •What protease inhibitors were used: no •What loading buffer was used: 5x sample buffer •Phosphatase inhibitors : no •Did you heat the samples: temperature and time: 100℃ , 5min AMOUNT OF PROTEIN LOADED 50ug ELECTROPHORESIS/GEL CONDITIONS •Reducing or non reducing gel: non-reducing •Reducing agent: •Gel percentage : 10% TRANSFER AND BLOCKING CONDITIONS Transfer conditions: (Type of membrane, Protein transfer verified): PVDF, 400mA, 1hr10min •Buffer: 3% BSA •Blocking agent: milk, BSA, serum, what percentage: 3% BSA •Incubation time: 1hr •Incubation temperature: RT SECONDARY ANTIBODY •Species: goat •Reacts against: rabbit •At what dilution(s) have you tested this antibody: 1/5000 •Incubation time: 1hr •Wash steps: 5min x 3 repeats, 10min x 2 repeats •Fluorochrome or enzyme conjugate: HRP •Do you know whether the problems you are experiencing come from the secondary? no HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? no

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Answer

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. Having reviewed your protocol and I have a few suggestions that may improve the results that you are currently seeing. Firstly, can I ask if there is a reason for not using a reducing agent? Adding DTT or b-mercaptoethanol can help to denature the protein by reducing the disulphide bonds in the protein. Additionally, I would strongly recommend using a protease inhibitor with the lysis buffer used as the M-Per lysis buffer does not appear to contain any itself. This should significantly reduce any degradation of the protein seen. Often altering the blocking agent used can also change the non-specific bands seen, in this case using 5% milk instead of 3% BSA may prove beneficial. I hope this information is helpful to you. Please do not hesitate to contact us if you continue to experience problems with this antibody, I will be happy to arrange a replacement of credit note for this product.

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