Overview

  • Product name

    Anti-Langerin antibody [EPR15863]
    See all Langerin primary antibodies
  • Description

    Rabbit monoclonal [EPR15863] to Langerin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Langerin aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UJ71

  • Positive control

    • IHC-P: Human skin tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. Major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. Protects against human immunodeficiency virus-1 (HIV-1) infection. Binds to high-mannose structures present on the envelope glycoprotein which is followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation.
  • Tissue specificity

    Exclusively expressed by Langerhans cells. Expressed in astrocytoma and malignant ependymoma, but not in normal brain tissues.
  • Involvement in disease

    Defects in CD207 are the cause of Birbeck granule deficiency (BIRGD) [MIM:613393]. It is a condition characterized by the absence of Birbeck granules in epidermal Langerhans cells. Despite the lack of Birbeck granules Langerhans cells are present in normal numbers and have normal morphologic characteristics and antigen-presenting capacity.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Domain

    The C-type lectin domain mediates dual recognition of both sulfated and mannosylated glycans.
  • Cellular localization

    Membrane. Found in Birbeck granules (BGs), which are organelles consisting of superimposed and zippered membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • C type lectin domain family 4 member K antibody
    • C-type lectin domain family 4 member K antibody
    • CD 207 antibody
    • CD207 antibody
    • CD207 antigen antibody
    • CD207 antigen, langerin antibody
    • CD207 molecule antibody
    • CLC4K_HUMAN antibody
    • CLEC 4K antibody
    • CLEC4K antibody
    • Langerhans cell specific c type lectin antibody
    • Langerin antibody
    • RGD1565913 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Langerin with ab192027 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilized PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Alexeev V CXCL12 as a Predictor of Vitiligo Activity and Disease Progression. J Invest Dermatol 137:1588-1590 (2017). IHC-P . Read more (PubMed: 28365260) »
  • Sundarasetty B  et al. Human Effector Memory T Helper Cells Engage with Mouse Macrophages and Cause Graft-versus-Host-Like Pathology in Skin of Humanized Mice Used in a Nonclinical Immunization Study. Am J Pathol 187:1380-1398 (2017). Read more (PubMed: 28432872) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry free floating
Sample
Human Tissue sections (Skin)
Specification
Skin

Abcam user community

Verified customer

Submitted May 09 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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