Anti-Lano antibody (ab127568)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Lano antibody
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DescriptionRabbit polyclonal to Lano
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 265 and 497 of Human Lano (UniProt Q9BTT6).
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Positive control
- A549, HeLa andcell lysates HepG2, HCT116 cell lysate; Human breast carcinoma tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7,00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab127568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 59 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificityExpressed strongly in testis and placenta, followed by heart, lung, kidney, thyroid, trachea, colon, prostate and pancreas.
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Sequence similaritiesContains 17 LRR (leucine-rich) repeats.
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Cellular localizationCytoplasm. Membrane. Localized at the basolateral side of epithelial cells.
- Information by UniProt
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Database links
- Entrez Gene: 55227 Human
- Entrez Gene: 214345 Mouse
- Omim: 608195 Human
- SwissProt: Q9BTT6 Human
- SwissProt: Q80VQ1 Mouse
- Unigene: 646997 Human
- Unigene: 657695 Human
- Unigene: 28534 Mouse
see all -
Alternative names
- dJ523E19.1 antibody
- FLJ10775 antibody
- FLJ11834 antibody
see all
Images
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Western blot - Anti-Lano antibody (ab127568)Anti-Lano antibody (ab127568) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 59 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lano antibody (ab127568)ab127568, at 1/250 dilution, staining Lano in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
References
ab127568 has not yet been referenced specifically in any publications.
