Anti-Lano antibody (ab127568)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Lano antibody -
Description
Rabbit polyclonal to Lano -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 265 and 497 of Human Lano (UniProt Q9BTT6).
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Positive control
- A549, HeLa andcell lysates HepG2, HCT116 cell lysate; Human breast carcinoma tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab127568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 59 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed strongly in testis and placenta, followed by heart, lung, kidney, thyroid, trachea, colon, prostate and pancreas. -
Sequence similarities
Contains 17 LRR (leucine-rich) repeats. -
Cellular localization
Cytoplasm. Membrane. Localized at the basolateral side of epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 55227 Human
- Entrez Gene: 214345 Mouse
- Omim: 608195 Human
- SwissProt: Q9BTT6 Human
- SwissProt: Q80VQ1 Mouse
- Unigene: 646997 Human
- Unigene: 657695 Human
- Unigene: 28534 Mouse
see all -
Alternative names
- dJ523E19.1 antibody
- FLJ10775 antibody
- FLJ11834 antibody
see all
Images
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Western blot - Anti-Lano antibody (ab127568)Anti-Lano antibody (ab127568) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 59 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lano antibody (ab127568)ab127568, at 1/250 dilution, staining Lano in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
References
ab127568 has not yet been referenced specifically in any publications.
