Anti-LAP2 alpha antibody [3A3] (ab66588)

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Key features and details

  • Mouse monoclonal [3A3] to LAP2 alpha
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-LAP2 alpha antibody [3A3]
    See all LAP2 alpha primary antibodies

  • Description

    Mouse monoclonal [3A3] to LAP2 alpha

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human LAP2 alpha aa 650-750 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • This antibody gave a positive signal in HeLa whole cell lysates and post-mitotic HeLa lysates.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab66588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa).
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Relevance

      Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure.

    • Cellular localization

      Nuclear

    • Database links

      • Alternative names

        • Isoforms beta/gamma antibody
        • Lamina associated polypeptide 2 antibody
        • LAP2 antibody
        • Thymopoietin antibody
        • Thymopoietin isoform alpha antibody
        • TMPO antibody
        • TP alpha antibody
        see all

      Images

      • Western blot - Anti-LAP2 alpha antibody [3A3] (ab66588)
        Western blot - Anti-LAP2 alpha antibody [3A3] (ab66588)
        All lanes : Anti-LAP2 alpha antibody [3A3] (ab66588) at 10 µg/ml

        Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 2 : HeLa (post mitotic stage) Whole Cell Lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Predicted band size: 75 kDa
        Observed band size: 77 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

      References (2)

      Publishing research using ab66588? Please let us know so that we can cite the reference in this datasheet.

      ab66588 has been referenced in 2 publications.

      • Liu S  et al. Nuclear envelope assembly defects link mitotic errors to chromothripsis. Nature 561:551-555 (2018). PubMed: 30232450
      • Qi R  et al. The lamin-A/C-LAP2a-BAF1 protein complex regulates mitotic spindle assembly and positioning. J Cell Sci 128:2830-41 (2015). PubMed: 26092935

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