Anti-LAP2 alpha antibody [3A3] (ab66588)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-LAP2 alpha antibody [3A3]
See all LAP2 alpha primary antibodies -
Description
Mouse monoclonal [3A3] to LAP2 alpha -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human LAP2 alpha aa 650-750 conjugated to keyhole limpet haemocyanin.
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Positive control
- This antibody gave a positive signal in HeLa whole cell lysates and post-mitotic HeLa lysates. IF/ICC: HeLa cells
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
3A3 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab66588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | Use a concentration of 1 µg/ml. | |
WB | Use a concentration of 10 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa). |
Target
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Relevance
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 7112 Human
- Omim: 188380 Human
- SwissProt: P42166 Human
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Alternative names
- Isoforms beta/gamma antibody
- Lamina associated polypeptide 2 antibody
- LAP2 antibody
see all
Images
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All lanes : Anti-LAP2 alpha antibody [3A3] (ab66588) at 10 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (post mitotic stage) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 75 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands. -
ICC/IF image of ab66588 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66588, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Datasheets and documents
References
This product has been referenced in:
- Qi R et al. The lamin-A/C-LAP2a-BAF1 protein complex regulates mitotic spindle assembly and positioning. J Cell Sci 128:2830-41 (2015). Read more (PubMed: 26092935) »