Overview

  • Product name

  • Description

    Rabbit polyclonal to LAP2 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human LAP2 alpha aa 200-250. The exact sequence is proprietary.
    Database link: P42166

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human breast carcinoma tissue; Mouse renal cell carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab226336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 75 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Isoforms beta/gamma antibody
    • Lamina associated polypeptide 2 antibody
    • LAP2 antibody
    • Thymopoietin antibody
    • Thymopoietin isoform alpha antibody
    • TMPO antibody
    • TP alpha antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for LAP2 alpha using ab226336 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.

  • All lanes : Anti-LAP2 alpha antibody (ab226336) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysates
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysates
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysates

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 75 kDa


    Exposure time: 30 seconds


    Cell lysates were prepared using NETN lysis buffer.

  • LAP2 alpha was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226336 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226336 at 1 µg/ml.

    Lane 1: ab226336 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

  • Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for LAP2 alpha using ab226336 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

References

ab226336 has not yet been referenced specifically in any publications.

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