Overview

  • Product name

    Anti-LAP2 alpha antibody - N-terminal
    See all LAP2 alpha primary antibodies
  • Description

    Rabbit polyclonal to LAP2 alpha - N-terminal
  • Host species

    Rabbit
  • Specificity

    At least three isoforms of LAP2 alpha are known to exist; ab189993 will detect all isoforms. ab189993 is predicted to not cross-react with other LEM domain-containing proteins.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human LAP2 alpha (N terminal). The exact sequence is proprietary. 19 amino acid peptide near the amino terminus. NP_003267.1.
    Database link: P42166

  • Positive control

    • Hela cells; Human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab189993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2.5 - 5 µg/ml.
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 75 kDa.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Relevance

    Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Isoforms beta/gamma antibody
    • Lamina associated polypeptide 2 antibody
    • LAP2 antibody
    • Thymopoietin antibody
    • Thymopoietin isoform alpha antibody
    • TMPO antibody
    • TP alpha antibody
    see all

Images

  • Immunocytochemical analysis of HeLa cells labeling LAP2 alpha with ab189993 at 2.5 µg/ml.

  • Immunofluorescent analysis of HeLa cells labeling LAP2 alpha with ab189993 at 5 µg/ml.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labeling LAP2 alpha with ab189993 at 2.5 µg/ml.

References

ab189993 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189993.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up