Anti-LAP2 alpha antibody - N-terminal (ab189993)
Key features and details
- Rabbit polyclonal to LAP2 alpha - N-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LAP2 alpha antibody - N-terminal
See all LAP2 alpha primary antibodies -
Description
Rabbit polyclonal to LAP2 alpha - N-terminal -
Host species
Rabbit -
Specificity
At least three isoforms of LAP2 alpha are known to exist; ab189993 will detect all isoforms. ab189993 is predicted to not cross-react with other LEM domain-containing proteins. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human LAP2 alpha (N terminal). The exact sequence is proprietary. 19 amino acid peptide near the amino terminus. NP_003267.1.
Database link: P42166 -
Positive control
- Hela cells; Human liver tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab189993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 2.5 - 5 µg/ml. | |
| WB | Use a concentration of 0.25 µg/ml. Predicted molecular weight: 75 kDa. | |
| IHC-P | Use a concentration of 2.5 µg/ml. |
Target
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Relevance
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 7112 Human
- Entrez Gene: 21917 Mouse
- Omim: 188380 Human
- SwissProt: P42166 Human
- SwissProt: Q61033 Mouse
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Alternative names
- Isoforms beta/gamma antibody
- Lamina associated polypeptide 2 antibody
- LAP2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody - N-terminal (ab189993)Immunocytochemical analysis of HeLa cells labeling LAP2 alpha with ab189993 at 2.5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody - N-terminal (ab189993)Immunofluorescent analysis of HeLa cells labeling LAP2 alpha with ab189993 at 5 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 alpha antibody - N-terminal (ab189993)Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labeling LAP2 alpha with ab189993 at 2.5 µg/ml.
Protocols
Datasheets and documents
References (0)
ab189993 has not yet been referenced specifically in any publications.
