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    lap2-antibody-ab185718.pdf

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Signal Transduction Cytoskeleton / ECM Basal Lamina
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Anti-LAP2 antibody (ab185718)

  • Datasheet
Submit a review Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
  • Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (ab185718)
  • Western blot - Anti-LAP2 antibody (ab185718)

Key features and details

  • Rabbit polyclonal to LAP2
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-LAP2 antibody
    See all LAP2 primary antibodies
  • Description

    Rabbit polyclonal to LAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human LAP2 aa 1 to the C-terminus.
    Database link: P42167

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Extracts from 22RV1, K562, HepG2, 293T, SH-SY5Y and H460 cell lines.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human LAP2 protein (ab151936)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/500 - 1/2000. Predicted molecular weight: 51 kDa.
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-P
1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/500 - 1/2000. Predicted molecular weight: 51 kDa.
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 7112 Human
    • Entrez Gene: 21917 Mouse
    • Omim: 188380 Human
    • SwissProt: P42166 Human
    • SwissProt: P42167 Human
    • SwissProt: Q61029 Mouse
    • SwissProt: Q61033 Mouse
    • Alternative names

      • Lamina associated polypeptide 2 antibody
      • LAP 2 antibody
      • LEM domain containing 4 antibody
      • LEMD4 antibody
      • Thymopoietin antibody
      • Thymopoietin isoform alpha antibody
      • Thymopoietin related peptide isoform alpha antibody
      • Thymopoietin related peptide isoforms beta/gamma antibody
      • Thymopoietin, isoforms beta/gamma antibody
      • TMPO antibody
      • TP alpha antibody
      • TP antibody
      • TP beta/gamma antibody
      • TPRP isoform alpha antibody
      • TPRP isoforms beta/gamma antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling LAP2 with ab185718 at 1/200. Magnification: 400x.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling LAP2 with ab185718 at 1/200. Magnification: 200x.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling LAP2 with ab185718 at 1/200. Magnification: 400x.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab185718)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling LAP2 with ab185718 at 1/200. Magnification: 400x.
    • Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (ab185718)
      Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (ab185718)
      Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185718. Blue DAPI for nuclear staining.
    • Western blot - Anti-LAP2 antibody (ab185718)
      Western blot - Anti-LAP2 antibody (ab185718)
      All lanes : Anti-LAP2 antibody (ab185718) at 1/500 dilution

      Lane 1 : Extract from 22RV1 cell line.
      Lane 2 : Extract from K562 cell line.
      Lane 3 : Extract from HepG2 cell line.
      Lane 4 : Extract from 293T cell line.
      Lane 5 : Extract from SH-SY5Y cell line.
      Lane 6 : Extract from H460 cell line.

      Predicted band size: 51 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab185718? Please let us know so that we can cite the reference in this datasheet.

    ab185718 has been referenced in 3 publications.

    • Chen Y  et al. The SUN1-SPDYA interaction plays an essential role in meiosis prophase I. Nat Commun 12:3176 (2021). PubMed: 34039995
    • Li Z  et al. TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO. Cancer Cell Int 20:294 (2020). PubMed: 32669970
    • Mirza AN  et al. LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription. Cell 176:198-212.e15 (2019). PubMed: 30503211

    Customer reviews and Q&As

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