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    lap2-antibody-ab223768.pdf

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Signal Transduction Cytoskeleton / ECM Basal Lamina
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Anti-LAP2 antibody (ab223768)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab223768)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab223768)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab223768)
  • Western blot - Anti-LAP2 antibody (ab223768)
  • Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (ab223768)

Key features and details

  • Rabbit polyclonal to LAP2
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Recombinant Human LAP2 protein (ab151936)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-LAP2 antibody
    See all LAP2 primary antibodies
  • Description

    Rabbit polyclonal to LAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human LAP2 aa 100-300.
    Database link: P42167

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human small intestine, prostate and lymph node tissues. WB: RT4, U-251 MG, NIH/3T3 and NBT-II cell lysates. ICC/IF: A431 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human LAP2 protein (ab151936)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab223768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 54 kDa.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 54 kDa.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Relevance

    Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 7112 Human
    • Entrez Gene: 21917 Mouse
    • Entrez Gene: 25359 Rat
    • Omim: 188380 Human
    • SwissProt: P42166 Human
    • SwissProt: P42167 Human
    • SwissProt: Q61029 Mouse
    • SwissProt: Q61033 Mouse
    • SwissProt: Q62733 Rat
    see all
  • Alternative names

    • Lamina associated polypeptide 2 antibody
    • LAP 2 antibody
    • LEM domain containing 4 antibody
    • LEMD4 antibody
    • Thymopoietin antibody
    • Thymopoietin isoform alpha antibody
    • Thymopoietin related peptide isoform alpha antibody
    • Thymopoietin related peptide isoforms beta/gamma antibody
    • Thymopoietin, isoforms beta/gamma antibody
    • TMPO antibody
    • TP alpha antibody
    • TP antibody
    • TP beta/gamma antibody
    • TPRP isoform alpha antibody
    • TPRP isoforms beta/gamma antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab223768)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab223768)

    Paraffin-embedded Human lymph node stained for LAP2 using ab223768 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab223768)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab223768)

    Paraffin-embedded Human prostate tissue stained for LAP2 using ab223768 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab223768)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab223768)

    Paraffin-embedded Human small intestine tissue stained for LAP2 using ab223768 at 1/200 dilution in immunohistochemical analysis.

  • Western blot - Anti-LAP2 antibody (ab223768)
    Western blot - Anti-LAP2 antibody (ab223768)
    All lanes : Anti-LAP2 antibody (ab223768) at 1/100 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 2 : NBT-II cell lysate

    Predicted band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (ab223768)
    Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (ab223768)

    PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for LAP2 (green) using ab223768 at 4 µg/ml in ICC/IF.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab223768? Please let us know so that we can cite the reference in this datasheet.

ab223768 has not yet been referenced specifically in any publications.

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