Key features and details
- Rabbit polyclonal to LAP2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-LAP2 antibody
See all LAP2 primary antibodies
DescriptionRabbit polyclonal to LAP2
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human LAP2 aa 225-275. The exact sequence is proprietary. (LAP2 beta).
Database link: P42167
- WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue; Mouse renal cell carcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226347 was affinity purified using an epitope specific to LAP2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 51 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceLamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
- Lamina associated polypeptide 2 antibody
- LAP 2 antibody
- LEM domain containing 4 antibody
All lanes : Anti-LAP2 antibody (ab226347) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
LAP2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226347 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226347 at 1 µg/ml.
Lane 1: ab226347 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for LAP2 using ab226347 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for LAP2 using ab226347 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226347 has not yet been referenced specifically in any publications.