Anti-LAP2 antibody (ab226348)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-LAP2 antibody
See all LAP2 primary antibodies -
Description
Rabbit polyclonal to LAP2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human LAP2 aa 350-400. The exact sequence is proprietary. (LAP2 beta).
Database link: P42167 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue; Mouse renal cell carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226348 was affinity purified using an epitope specific to LAP2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 51 kDa. |
Target
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Relevance
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 7112 Human
- Entrez Gene: 21917 Mouse
- Entrez Gene: 25359 Rat
- Omim: 188380 Human
- SwissProt: P42166 Human
- SwissProt: P42167 Human
- SwissProt: Q61029 Mouse
- SwissProt: Q61033 Mouse
see all -
Alternative names
- Lamina associated polypeptide 2 antibody
- LAP 2 antibody
- LEM domain containing 4 antibody
see all
Images
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Western blot - Anti-LAP2 antibody (ab226348)All lanes : Anti-LAP2 antibody (ab226348) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-LAP2 antibody (ab226348)LAP2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226348 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226348 at 1 µg/ml.
Lane 1: ab226348 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab226348)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for LAP2 using ab226348 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab226348)Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for LAP2 using ab226348 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
Datasheets and documents
References
ab226348 has not yet been referenced specifically in any publications.
