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  1. Link

    lap2-antibody-ab226348.pdf

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Signal Transduction Cytoskeleton / ECM Basal Lamina
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Anti-LAP2 antibody (ab226348)

  • Datasheet
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Western blot - Anti-LAP2 antibody (ab226348)
  • Immunoprecipitation - Anti-LAP2 antibody (ab226348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab226348)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab226348)

Key features and details

  • Rabbit polyclonal to LAP2
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Protein
Recombinant Human LAP2 protein (ab151936)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)

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Overview

  • Product name

    Anti-LAP2 antibody
    See all LAP2 primary antibodies
  • Description

    Rabbit polyclonal to LAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human LAP2 aa 350-400. The exact sequence is proprietary. (LAP2 beta).
    Database link: P42167

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue; Mouse renal cell carcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226348 was affinity purified using an epitope specific to LAP2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class V
    • Lamins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human LAP2 protein (ab151936)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab226348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at 2-10 µg/mg of lysate.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
Notes
IP
Use at 2-10 µg/mg of lysate.
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.

Target

  • Relevance

    Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 7112 Human
    • Entrez Gene: 21917 Mouse
    • Entrez Gene: 25359 Rat
    • Omim: 188380 Human
    • SwissProt: P42166 Human
    • SwissProt: P42167 Human
    • SwissProt: Q61029 Mouse
    • SwissProt: Q61033 Mouse
    • SwissProt: Q62733 Rat
    see all
  • Alternative names

    • Lamina associated polypeptide 2 antibody
    • LAP 2 antibody
    • LEM domain containing 4 antibody
    • LEMD4 antibody
    • Thymopoietin antibody
    • Thymopoietin isoform alpha antibody
    • Thymopoietin related peptide isoform alpha antibody
    • Thymopoietin related peptide isoforms beta/gamma antibody
    • Thymopoietin, isoforms beta/gamma antibody
    • TMPO antibody
    • TP alpha antibody
    • TP antibody
    • TP beta/gamma antibody
    • TPRP isoform alpha antibody
    • TPRP isoforms beta/gamma antibody
    see all

Images

  • Western blot - Anti-LAP2 antibody (ab226348)
    Western blot - Anti-LAP2 antibody (ab226348)
    All lanes : Anti-LAP2 antibody (ab226348) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-LAP2 antibody (ab226348)
    Immunoprecipitation - Anti-LAP2 antibody (ab226348)

    LAP2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226348 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226348 at 1 µg/ml.

    Lane 1: ab226348 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab226348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab226348)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for LAP2 using ab226348 at 1/1000 dilution in immunohistochemical analysis.

    Detection: DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab226348)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (ab226348)

    Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for LAP2 using ab226348 at 1/1000 dilution in immunohistochemical analysis.

    Detection: DAB staining.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab226348? Please let us know so that we can cite the reference in this datasheet.

ab226348 has been referenced in 1 publication.

  • Zhou H  et al. Downregulation of thymopoietin by miR-139-5p suppresses cell proliferation and induces cell cycle arrest/apoptosis in pancreatic ductal adenocarcinoma. Oncol Lett 18:3443-3452 (2019). PubMed: 31516562

Customer reviews and Q&As

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