Overview

  • Product name

  • Description

    Rabbit polyclonal to LAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LAP2 aa 300-350. The exact sequence is proprietary. Isoform beta.
    Database link: P42167-1

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab226352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Lamina associated polypeptide 2 antibody
    • LAP 2 antibody
    • LEM domain containing 4 antibody
    • LEMD4 antibody
    • Thymopoietin antibody
    • Thymopoietin isoform alpha antibody
    • Thymopoietin related peptide isoform alpha antibody
    • Thymopoietin related peptide isoforms beta/gamma antibody
    • Thymopoietin, isoforms beta/gamma antibody
    • TMPO antibody
    • TP alpha antibody
    • TP antibody
    • TP beta/gamma antibody
    • TPRP isoform alpha antibody
    • TPRP isoforms beta/gamma antibody
    see all

Images

  • All lanes : Anti-LAP2 antibody (ab226352) at 0.4 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 75 kDa


    Exposure time: 30 seconds
  • LAP2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226352 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using a different rabbit anti-LAP2 antibody at 1 µg/ml.

    Lane 1: ab226352 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for LAP2 using ab226352 at 1/1000 dilution in immunohistochemical analysis.

    Detection: DAB staining.

References

ab226352 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226352.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up