Key features and details
- Rabbit polyclonal to LAP2
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LAP2 antibody
See all LAP2 primary antibodies
DescriptionRabbit polyclonal to LAP2
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human LAP2 aa 404-454. The exact sequence is proprietary.
Database link: P42167
- WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma and breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/2500. Predicted molecular weight: 75 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceLamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
- Lamina associated polypeptide 2 antibody
- LAP 2 antibody
- LEM domain containing 4 antibody
All lanes : Anti-LAP2 antibody (ab226354) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for LAP2 using ab226354 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
LAP2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226354 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226354 at 1 µg/ml.
Lane 1: ab226354 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for LAP2 using ab226354 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226354 has not yet been referenced specifically in any publications.