Anti-LAP3 antibody [EPR10330] Recombinant (ab154809)

Product name

Anti-LAP3 antibody [EPR10330]
See all LAP3 primary antibodies
Description

Rabbit monoclonal [EPR10330] to LAP3
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivity

Reacts with: Rat, Human
Immunogen

Synthetic peptide within Human LAP3 aa 1-100. The exact sequence is proprietary.
(Peptide available as ab200873)
Positive control
- WB: Human fetal liver, 293T, HepG2 and HeLa lysates. IHC-P: Human kidney and ovarian carcinoma tissues. Flow Cyt: 293T cells.
General notes

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10330
Isotype

IgG
Research areas

Positive Controls
Related Products
- Recombinant Human LAP3 protein (ab132775)

Our Abpromise guarantee covers the use of ab154809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application

Abreviews

Notes

WB

1/1000 - 1/10000. Predicted molecular weight: 56 kDa.

IHC-P

1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cyt

1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for ICC/IF or IP.

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
Sequence similarities

Belongs to the peptidase M17 family.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P28838 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 51056 Human
- Entrez Gene: 289668 Rat
- Omim: 170250 Human
- SwissProt: P28838 Human
- SwissProt: Q68FS4 Rat
- Unigene: 570791 Human
- Unigene: 99790 Rat
Alternative names
- AMPL_HUMAN antibody
- Cytosol aminopeptidase antibody
- epididymis secretory protein Li 106 antibody
- HEL-S-106 antibody
- LAP 3 antibody
- LAP antibody
- LAP-3 antibody
- Lap3 antibody
- LAPEP antibody
- Leucine aminopeptidase 3 antibody
- Leucyl aminopeptidase antibody
- PEPS antibody
- Peptidase S antibody
- Proline aminopeptidase antibody
- Prolyl aminopeptidase antibody
see all

Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)

All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : 293T lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin-embedded kidney tissue labeling LAP3 with ab154809 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin-embedded ovarian carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution.
Flow Cytometry - Anti-LAP3 antibody [EPR10330] (ab154809)

Flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue using ab154809 showing +ve staining.

Datasheet

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

ab154809 has not yet been referenced specifically in any publications.

Publishing research using ab154809? Please let us know so that we can cite the reference in this datasheet.

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Anti-LAP3 antibody [EPR10330] (ab154809)

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