Recombinant
RabMAb

Recombinant Anti-LAP3 antibody [EPR10330] (ab154809)

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Overview

  • Product name

    Anti-LAP3 antibody [EPR10330]
    See all LAP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10330] to LAP3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human LAP3 aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab200873)

  • Positive control

    • WB: Human fetal liver, 293T, HepG2 and HeLa lysates. IHC-P: Human kidney and ovarian carcinoma tissues. Flow Cyt: 293T cells.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab154809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Function

      Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.

    • Sequence similarities

      Belongs to the peptidase M17 family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • AMPL_HUMAN antibody
        • Cytosol aminopeptidase antibody
        • epididymis secretory protein Li 106 antibody
        • HEL-S-106 antibody
        • LAP 3 antibody
        • LAP antibody
        • LAP-3 antibody
        • Lap3 antibody
        • LAPEP antibody
        • Leucine aminopeptidase 3 antibody
        • Leucyl aminopeptidase antibody
        • PEPS antibody
        • Peptidase S antibody
        • Proline aminopeptidase antibody
        • Prolyl aminopeptidase antibody
        see all

      Images

      • Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)
        Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)
        All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : 293T lysate
        Lane 3 : HepG2 lysate
        Lane 4 : HeLa lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 56 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

        Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling LAP3 with ab154809 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

        Immunohistochemical analysis of paraffin-embedded ovarian human carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Flow Cytometry - Anti-LAP3 antibody [EPR10330] (ab154809)
        Flow Cytometry - Anti-LAP3 antibody [EPR10330] (ab154809)
        Flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

        Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

        Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

        Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

        Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue using ab154809 showing +ve staining.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Wu H  et al. A new method to evaluate the enzyme-suppressing activity of a leucine aminopeptidase 3 inhibitor. Drug Discov Ther 13:17-21 (2019). Read more (PubMed: 30880317) »
      • Yang Q  et al. Proteomic profiling of antibody-inducing immunogens in tumor tissue identifies PSMA1, LAP3, ANXA3, and maspin as colon cancer markers. Oncotarget 9:3996-4019 (2018). Read more (PubMed: 29423100) »
      See all 2 Publications for this product

      Publishing research using ab154809? Please let us know so that we can cite the reference in this datasheet.

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