Recombinant Anti-LAP3 antibody [EPR10330] KO Tested (ab154809)

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Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10330] to LAP3
Suitable for: Flow Cyt (Intra), WB, IHC-P
Knockout validated
Reacts with: Human

Carrier Free

Product name

Anti-LAP3 antibody [EPR10330]
See all LAP3 primary antibodies
Description

Rabbit monoclonal [EPR10330] to LAP3
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivity

Reacts with: Human
Predicted to work with: Rat
Immunogen

Synthetic peptide within Human LAP3 aa 1-100. The exact sequence is proprietary.
(Peptide available as ab200873)
Positive control
- WB: A549, 293T, HepG2 and HeLa cell lysates; Human fetal liver lysate. IHC-P: Human kidney and ovarian carcinoma tissues. Flow Cyt (intra): 293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10330
Isotype

IgG
Research areas

Alternative Versions
- Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human LAP3 knockout A549 cell line (ab266986)
KO cell lysates
- Human LAP3 knockout A549 cell lysate (ab258023)
Positive Controls
- HeLa whole cell lysate (ab150035)
- HeLa whole cell lysate (ab29545)
Recombinant Protein
- Recombinant Human LAP3 protein (ab202613)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Notes
Flow Cyt (Intra) 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for ICC/IF or IP.

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
Sequence similarities

Belongs to the peptidase M17 family.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P28838 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 51056 Human
- Entrez Gene: 289668 Rat
- Omim: 170250 Human
- SwissProt: P28838 Human
- SwissProt: Q68FS4 Rat
- Unigene: 570791 Human
- Unigene: 99790 Rat
Alternative names
- AMPL_HUMAN antibody
- Cytosol aminopeptidase antibody
- epididymis secretory protein Li 106 antibody
- HEL-S-106 antibody
- LAP 3 antibody
- LAP antibody
- LAP-3 antibody
- Lap3 antibody
- LAPEP antibody
- Leucine aminopeptidase 3 antibody
- Leucyl aminopeptidase antibody
- PEPS antibody
- Peptidase S antibody
- Proline aminopeptidase antibody
- Prolyl aminopeptidase antibody
see all

Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)

All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : LAP3 knockout A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Lanes 1-4: Merged signal (red and green). Green - ab154809 observed at 56 kDa. Red - loading control ab8245 observed at 36 kDa.

ab154809 Anti-LAP3 antibody [EPR10330] was shown to specifically react with LAP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266986 (knockout cell lysate ab258023) was used. Wild-type and LAP3 knockout samples were subjected to SDS-PAGE. ab154809 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling LAP3 with ab154809 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-LAP3 antibody [EPR10330] (ab154809)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).
Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)

All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : 293T lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin-embedded ovarian human carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-LAP3 antibody [EPR10330] (ab154809)

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab154809? Please let us know so that we can cite the reference in this datasheet.

ab154809 has been referenced in 2 publications.

Wu H et al. A new method to evaluate the enzyme-suppressing activity of a leucine aminopeptidase 3 inhibitor. Drug Discov Ther 13:17-21 (2019). PubMed: 30880317
Yang Q et al. Proteomic profiling of antibody-inducing immunogens in tumor tissue identifies PSMA1, LAP3, ANXA3, and maspin as colon cancer markers. Oncotarget 9:3996-4019 (2018). PubMed: 29423100

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (2)

Customer reviews and Q&As