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    lap3-antibody-epr10330-ab154809.pdf

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Signal Transduction Metabolism Amino Acids
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-LAP3 antibody [EPR10330] (ab154809)

  • Datasheet
  • SDS
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Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
  • Flow Cytometry (Intracellular) - Anti-LAP3 antibody [EPR10330] (ab154809)
  • Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
  • Anti-LAP3 antibody [EPR10330] (ab154809)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10330] to LAP3
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Primary
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Anti-Annexin A3/ANXA3 antibody [6#] (ab239582)
Knockout
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Human LAP3 knockout A549 cell line (ab266986)

View more associated products

Overview

  • Product name

    Anti-LAP3 antibody [EPR10330]
    See all LAP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR10330] to LAP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human LAP3 aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab200873)

  • Positive control

    • WB: A549, 293T, HepG2 and HeLa cell lysates; Human fetal liver lysate. IHC-P: Human kidney and ovarian carcinoma tissues. Flow Cyt (intra): 293T cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10330
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-LAP3 antibody [EPR10330] - BSA and Azide free (ab249116)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human LAP3 knockout A549 cell line (ab266986)
  • KO cell lysates

    • Human LAP3 knockout A549 cell lysate (ab258023)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human LAP3 protein (ab202613)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
  • Sequence similarities

    Belongs to the peptidase M17 family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P28838 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51056 Human
    • Entrez Gene: 289668 Rat
    • Omim: 170250 Human
    • SwissProt: P28838 Human
    • SwissProt: Q68FS4 Rat
    • Unigene: 570791 Human
    • Unigene: 99790 Rat
    • Alternative names

      • AMPL_HUMAN antibody
      • Cytosol aminopeptidase antibody
      • epididymis secretory protein Li 106 antibody
      • HEL-S-106 antibody
      • LAP 3 antibody
      • LAP antibody
      • LAP-3 antibody
      • Lap3 antibody
      • LAPEP antibody
      • Leucine aminopeptidase 3 antibody
      • Leucyl aminopeptidase antibody
      • PEPS antibody
      • Peptidase S antibody
      • Proline aminopeptidase antibody
      • Prolyl aminopeptidase antibody
      see all

    Images

    • Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)
      Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)
      All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

      Lane 1 : Wild-type A549 cell lysate
      Lane 2 : LAP3 knockout A549 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 56 kDa
      Observed band size: 56 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab154809 observed at 56 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab154809 Anti-LAP3 antibody [EPR10330] was shown to specifically react with LAP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266986 (knockout cell lysate ab258023) was used. Wild-type and LAP3 knockout samples were subjected to SDS-PAGE. ab154809 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling LAP3 with ab154809 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-LAP3 antibody [EPR10330] (ab154809)
      Flow Cytometry (Intracellular) - Anti-LAP3 antibody [EPR10330] (ab154809)

      Intracellular flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).

       

    • Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)
      Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)
      All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : 293T lysate
      Lane 3 : HepG2 lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 56 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

      Immunohistochemical analysis of paraffin-embedded ovarian human carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

      Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

      Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

      Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

      Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue using ab154809 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-LAP3 antibody [EPR10330] (ab154809)
      Anti-LAP3 antibody [EPR10330] (ab154809)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab154809? Please let us know so that we can cite the reference in this datasheet.

    ab154809 has been referenced in 2 publications.

    • Wu H  et al. A new method to evaluate the enzyme-suppressing activity of a leucine aminopeptidase 3 inhibitor. Drug Discov Ther 13:17-21 (2019). PubMed: 30880317
    • Yang Q  et al. Proteomic profiling of antibody-inducing immunogens in tumor tissue identifies PSMA1, LAP3, ANXA3, and maspin as colon cancer markers. Oncotarget 9:3996-4019 (2018). PubMed: 29423100

    Customer reviews and Q&As

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