Name: Anti-LAP3 antibody [EPR10330]
Brand: RabMAb
SKU: ab154809

Product name

Anti-LAP3 antibody [EPR10330]
See all LAP3 primary antibodies
Description

Rabbit monoclonal [EPR10330] to LAP3
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivity

Reacts with: Rat, Human
Immunogen

Synthetic peptide within Human LAP3 aa 1-100. The exact sequence is proprietary.
(Peptide available as ab200873)
Positive control
- WB: Human fetal liver, 293T, HepG2 and HeLa lysates. IHC-P: Human kidney and ovarian carcinoma tissues. Flow Cyt: 293T cells.
General notes

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10330
Isotype

IgG
Research areas

Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Related Products
- Recombinant Human LAP3 protein (ab132775)

Our Abpromise guarantee covers the use of ab154809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

1/1000 - 1/10000. Predicted molecular weight: 56 kDa.

IHC-P

1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cyt

1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for ICC/IF or IP.

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
Sequence similarities

Belongs to the peptidase M17 family.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P28838 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 51056 Human
- Entrez Gene: 289668 Rat
- Omim: 170250 Human
- SwissProt: P28838 Human
- SwissProt: Q68FS4 Rat
- Unigene: 570791 Human
- Unigene: 99790 Rat
Alternative names
- AMPL_HUMAN antibody
- Cytosol aminopeptidase antibody
- epididymis secretory protein Li 106 antibody
- HEL-S-106 antibody
- LAP 3 antibody
- LAP antibody
- LAP-3 antibody
- Lap3 antibody
- LAPEP antibody
- Leucine aminopeptidase 3 antibody
- Leucyl aminopeptidase antibody
- PEPS antibody
- Peptidase S antibody
- Proline aminopeptidase antibody
- Prolyl aminopeptidase antibody
see all

Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)

All lanes : Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : 293T lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin-embedded kidney tissue labeling LAP3 with ab154809 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin-embedded ovarian carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution.
Flow Cytometry - Anti-LAP3 antibody [EPR10330] (ab154809)

Flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue using ab154809 showing +ve staining.

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Datasheet

This product has been referenced in:

Yang Q et al. Proteomic profiling of antibody-inducing immunogens in tumor tissue identifies PSMA1, LAP3, ANXA3, and maspin as colon cancer markers. Oncotarget 9:3996-4019 (2018). Read more (PubMed: 29423100) »

See 1 Publication for this product

Publishing research using ab154809? Please let us know so that we can cite the reference in this datasheet.

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Anti-LAP3 antibody [EPR10330] (ab154809)

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