Product nameAnti-LAP3 antibody - N-terminal
See all LAP3 primary antibodies
DescriptionRabbit polyclonal to LAP3 - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
Recombinant fragment within Human LAP3 (N terminal). The exact sequence is proprietary.
Database link: P28838
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 56 kDa.|
|IP||1/100 - 1/500.|
FunctionPresumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
Sequence similaritiesBelongs to the peptidase M17 family.
- Information by UniProt
- AMPL_HUMAN antibody
- Cytosol aminopeptidase antibody
- epididymis secretory protein Li 106 antibody
LAP3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg of ab229577. Western blot was performed form the immunoprecipitate using ab229577.
Lane 1: HEK-293T whole cell extract (input).
Lane 2: Control IgG IP in HEK-293T whole cell extract.
Lane 3: ab229577 IP in HEK-293T whole cell extract.
All lanes : Anti-LAP3 antibody - N-terminal (ab229577) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
10% SDS-PAGE gel.
ab229577 has not yet been referenced specifically in any publications.