Overview

  • Product name

    Anti-LAP3 antibody - N-terminal
    See all LAP3 primary antibodies
  • Description

    Rabbit polyclonal to LAP3 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human LAP3 (N terminal). The exact sequence is proprietary.
    Database link: P28838

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
  • Sequence similarities

    Belongs to the peptidase M17 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMPL_HUMAN antibody
    • Cytosol aminopeptidase antibody
    • epididymis secretory protein Li 106 antibody
    • HEL-S-106 antibody
    • LAP 3 antibody
    • LAP antibody
    • LAP-3 antibody
    • Lap3 antibody
    • LAPEP antibody
    • Leucine aminopeptidase 3 antibody
    • Leucyl aminopeptidase antibody
    • PEPS antibody
    • Peptidase S antibody
    • Proline aminopeptidase antibody
    • Prolyl aminopeptidase antibody
    see all

Images

  • LAP3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg of ab229577. Western blot was performed form the immunoprecipitate using ab229577.

    Lane 1: HEK-293T whole cell extract (input).

    Lane 2: Control IgG IP in HEK-293T whole cell extract.

    Lane 3: ab229577 IP in HEK-293T whole cell extract.

  • All lanes : Anti-LAP3 antibody - N-terminal (ab229577) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa



    10% SDS-PAGE gel.

References

ab229577 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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