Key features and details
- Rabbit polyclonal to LAP3 - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LAP3 antibody - N-terminal
See all LAP3 primary antibodies
DescriptionRabbit polyclonal to LAP3 - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
Recombinant fragment within Human LAP3 (N terminal). The exact sequence is proprietary.
Database link: P28838
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 56 kDa.|
|IP||1/100 - 1/500.|
FunctionPresumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
Sequence similaritiesBelongs to the peptidase M17 family.
- Information by UniProt
- AMPL_HUMAN antibody
- Cytosol aminopeptidase antibody
- epididymis secretory protein Li 106 antibody
LAP3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg of ab229577. Western blot was performed form the immunoprecipitate using ab229577.
Lane 1: HEK-293T whole cell extract (input).
Lane 2: Control IgG IP in HEK-293T whole cell extract.
Lane 3: ab229577 IP in HEK-293T whole cell extract.
All lanes : Anti-LAP3 antibody - N-terminal (ab229577) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229577 has not yet been referenced specifically in any publications.