Key features and details
- Rabbit polyclonal to LARG
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LARG antibody
See all LARG primary antibodies
DescriptionRabbit polyclonal to LARG
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residues 1350 and 1400 of human LARG (NP_056128.1)
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86095 was affinity purified using an epitope specific to LARG immobilized on solid support.
Our Abpromise guarantee covers the use of ab86095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 173 kDa (predicted molecular weight: 173 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
Tissue specificityUbiquitously expressed. Isoform 2 is found in jejunum and testis.
Involvement in diseaseA chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with KMT2A/MLL1.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PDZ (DHR) domain.
Contains 1 PH domain.
Contains 1 RGSL (RGS-like) domain.
Cellular localizationCytoplasm. Membrane. Translocated to the membrane upon stimulation.
- Information by UniProt
- ARHGC_HUMAN antibody
- ARHGEF12 antibody
- KIAA0382 antibody
All lanes : Anti-LARG antibody (ab86095) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 173 kDa
Observed band size: 173 kDa
Exposure time: 3 minutes
Detection of LARG by Western Blot of Immunprecipitate.
ab86095 at 1µg/ml staining LARG in HeLa whole cell lysate immunoprecipitated using ab86095 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab86095 has been referenced in 1 publication.
- Osborne LD et al. TGF-ß regulates LARG and GEF-H1 during EMT to affect stiffening response to force and cell invasion. Mol Biol Cell 25:3528-40 (2014). WB . PubMed: 25143398