Anti-LARP1 antibody (ab245635)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-LARP1 antibody
See all LARP1 primary antibodies -
Description
Rabbit polyclonal to LARP1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human LARP1 aa 575-625. The exact sequence is proprietary. NP_291029.2
Database link: Q6PKG0 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245635 was affinity purified using an epitope specific to LARP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab245635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 124 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
LARP1 belongs to the LARP family and contains 1 HTH La-type RNA-binding domain. -
Database links
- Entrez Gene: 23367 Human
- Omim: 612059 Human
- SwissProt: Q6PKG0 Human
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Alternative names
- 1810024J12Rik antibody
- 3110040D16Rik antibody
- KIAA0731 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LARP1 antibody (ab245635)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for LARP1 using ab245635 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
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Western blot - Anti-LARP1 antibody (ab245635)All lanes : Anti-LARP1 antibody (ab245635) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-LARP1 antibody (ab245635)LARP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245635 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245635 at 1 µg/ml.
Lane 1: ab245635 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
Datasheets and documents
References
ab245635 has not yet been referenced specifically in any publications.
