Overview

  • Product name

  • Description

    Rabbit polyclonal to LARP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 275 and 325 of Human LARP1 (NP_291029.2)

  • Positive control

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    LARP1 belongs to the LARP family and contains 1 HTH La-type RNA-binding domain.
  • Database links

  • Alternative names

    • 1810024J12Rik antibody
    • 3110040D16Rik antibody
    • KIAA0731 antibody
    • La related protein antibody
    • La ribonucleoprotein domain family, member 1 antibody
    • LARP antibody
    • MGC19556 antibody
    • mKIAA0731 antibody
    • rCG_32791 antibody
    • RGD1306683 antibody
    • RP23-293I3.7 antibody
    see all

Images

  • All lanes : Anti-LARP1 antibody (ab86359) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 124 kDa


    Exposure time: 10 seconds
  • Ab86359 staining LARP1 in human colon carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde- fixed, paraffin embedded tissue sections). Samples were incubated with primary antibody at 1:200 dilution (1μg/ml). 

  • All lanes : Anti-LARP1 antibody (ab86359) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 124 kDa


    Exposure time: 10 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling LARP1 with ab86359 at 1/200 (1µg/ml). Detection: DAB.

References

This product has been referenced in:

  • Xu Z  et al. LARP1 is regulated by the XIST/miR-374a axis and functions as an oncogene in non-small cell lung carcinoma. Oncol Rep 38:3659-3667 (2017). Read more (PubMed: 29039571) »
  • Müller AC  et al. Identifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry. Sci Rep 6:28107 (2016). Read more (PubMed: 27346722) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (PBMC)
Permeabilization
No
Gating Strategy
Live cell population
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: Isolation of PBMC from whole blood
Sample buffer: PBS
Fixation
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 27 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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