Product nameAnti-LARP4 antibody
See all LARP4 primary antibodies
DescriptionRabbit polyclonal to LARP4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human LARP4 aa 674-724. The exact sequence is proprietary.
Database link: Q71RC2
- WB: HEK-293T, HeLa, Jurkat and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to LARP4 immobilized on solid support
Our Abpromise guarantee covers the use of ab241489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 81 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceLARP4 contains one HTH La-type RNA-binding domain and one RRM (RNA recognition motif) domain. There are four named isoforms.
- c Mpl binding protein antibody
- La related protein 4 antibody
- La ribonucleoprotein domain family member 4 antibody
- PP13296 antibody
All lanes : Anti-LARP4 antibody (ab241489) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 30 seconds
LARP4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP; 20% of IP loaded) using ab241489 at 6 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab241489 at 1 µg/ml.
Lane 1: ab241489 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence withan exposure time of 10 seconds.
ab241489 has not yet been referenced specifically in any publications.