Key features and details
- Rabbit polyclonal to LARP4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-LARP4 antibody
See all LARP4 primary antibodies
DescriptionRabbit polyclonal to LARP4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human LARP4 aa 674-724. The exact sequence is proprietary.
Database link: Q71RC2
- WB: HEK-293T, HeLa, Jurkat and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to LARP4 immobilized on solid support
Our Abpromise guarantee covers the use of ab241489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 81 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceLARP4 contains one HTH La-type RNA-binding domain and one RRM (RNA recognition motif) domain. There are four named isoforms.
- c Mpl binding protein antibody
- La related protein 4 antibody
- La ribonucleoprotein domain family member 4 antibody
- PP13296 antibody
All lanes : Anti-LARP4 antibody (ab241489) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 30 seconds
LARP4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP; 20% of IP loaded) using ab241489 at 6 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab241489 at 1 µg/ml.
Lane 1: ab241489 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence withan exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241489 has been referenced in 1 publication.
- Lin W et al. Up-regulation of circ_LARP4 suppresses cell proliferation and migration in ovarian cancer by regulating miR-513b-5p/LARP4 axis. Cancer Cell Int 20:5 (2020). PubMed: 31911757