Overview

  • Product name

  • Description

    Rabbit polyclonal to LARP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human LARP4 aa 674-724. The exact sequence is proprietary.
    Database link: Q71RC2

  • Positive control

    • WB: HEK-293T, HeLa, Jurkat and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to LARP4 immobilized on solid support
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 81 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    LARP4 contains one HTH La-type RNA-binding domain and one RRM (RNA recognition motif) domain. There are four named isoforms.
  • Database links

  • Alternative names

    • c Mpl binding protein antibody
    • La related protein 4 antibody
    • La ribonucleoprotein domain family member 4 antibody
    • PP13296 antibody

Images

  • All lanes : Anti-LARP4 antibody (ab241489) at 0.4 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 81 kDa


    Exposure time: 30 seconds
  • LARP4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP; 20% of IP loaded) using ab241489 at 6 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab241489 at 1 µg/ml.

    Lane 1: ab241489 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG in HEK-293T whole cell lysate.

    Detection: Chemiluminescence withan exposure time of 10 seconds.

     

References

ab241489 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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