Product nameAnti-LARP4B antibody
DescriptionRabbit polyclonal to LARP4B
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cat, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat and TCMK-1 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab197085 was affinity purified using an epitope specific to LARP4B immobilized on solid support.
Our Abpromise guarantee covers the use of ab197085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 81 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesContains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- A630096F19 antibody
- AI256361 antibody
- D13Wsu64e antibody
All lanes : Anti-LARP4B antibody (ab197085) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
Detection of LARP4B in Immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197085 at 6 µg/mg lysate for IP (Lane 1) and at 0.1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab197085 has been referenced in 1 publication.
- Yin W et al. MicroRNA-106b functions as an oncogene and regulates tumor viability and metastasis by targeting LARP4B in prostate cancer. Mol Med Rep 20:951-958 (2019). PubMed: 31173237