Product nameAnti-LARP7 antibody
See all LARP7 primary antibodies
DescriptionRabbit polyclonal to LARP7
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Gorilla, Orangutan
- HeLa, 293T, Jurkat and NIH 3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7.0 to 8.0
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab134746 is purified using an epitope specific to LARP7 immobilized on solid support.
Our Abpromise guarantee covers the use of ab134746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionNegative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.
Sequence similaritiesContains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
- DKFZp564K112 antibody
- HDCMA18P antibody
- La related protein 7 antibody
All lanes : Anti-LARP7 antibody (ab134746) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 10 seconds
Western Blot Gel 4-20%
Detection of LARP7 by Western Blot of Immunprecipitate.
Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134746 at 6 µg/mg lysate for IP.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab134746 has not yet been referenced specifically in any publications.