Overview

  • Product name

  • Description

    Rabbit polyclonal to LARP7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human LARP7 (NP_056269.1).

  • Positive control

    • HeLa, 293T, Jurkat and NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab134746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.
  • Sequence similarities

    Contains 1 HTH La-type RNA-binding domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp564K112 antibody
    • HDCMA18P antibody
    • La related protein 7 antibody
    • La ribonucleoprotein domain family member 7 antibody
    • La ribonucleoprotein domain family member 7 antibody
    • La-related protein 7 antibody
    • LARP 7 antibody
    • larp7 antibody
    • LARP7_HUMAN antibody
    • MGC104360 antibody
    • P TEFb interaction protein for 7SK stability antibody
    • P-TEFb-interaction protein for 7SK stability antibody
    • PIP7S antibody
    see all

Images

  • All lanes : Anti-LARP7 antibody (ab134746) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa


    Exposure time: 10 seconds


    Western Blot Gel 4-20%
  • Detection of LARP7 by Western Blot of Immunprecipitate.
    Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134746 at 6 µg/mg lysate for IP.
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab134746 has not yet been referenced specifically in any publications.

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