Overview

  • Product name

  • Description

    Rabbit polyclonal to LARP7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within the C terminal amino acids 532-582 of Human LARP7 (NP_056269.1).

  • Positive control

    • Whole cell lysate from HeLa, 293T, Jurkat and mouse NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab134757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.
  • Sequence similarities

    Contains 1 HTH La-type RNA-binding domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp564K112 antibody
    • HDCMA18P antibody
    • La related protein 7 antibody
    • La ribonucleoprotein domain family member 7 antibody
    • La ribonucleoprotein domain family member 7 antibody
    • La-related protein 7 antibody
    • LARP 7 antibody
    • larp7 antibody
    • LARP7_HUMAN antibody
    • MGC104360 antibody
    • P TEFb interaction protein for 7SK stability antibody
    • P-TEFb-interaction protein for 7SK stability antibody
    • PIP7S antibody
    see all

Images

  • All lanes : Anti-LARP7 antibody (ab134757) at 0.1 µg/ml

    Lane 1 : Whole cell
    lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell
    lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell
    lysate from 293T cells at 50 µg
    Lane 4 : Whole cell
    lysate from Jurkat cells at 50 µg
    Lane 5 : Whole cell
    lysate from mouse NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa


    Exposure time: 30 seconds


    Gel concentration 4-20%
  • Lane 1: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of LARP7.
    Lane 2: Detection of Human LARP7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134757 at 6 µg/mg lysate for IP.
    Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

    Subsequent Western blot detection used ab134757 at 1 µg/ml.
    Gel concentration 4-20%

    Developed using the ECL technique with an exposure time of 10 seconds.

References

ab134757 has not yet been referenced specifically in any publications.

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