Product nameAnti-LARP7 antibody
See all LARP7 primary antibodies
DescriptionRabbit polyclonal to LARP7
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
- Whole cell lysate from HeLa, 293T, Jurkat and mouse NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7.0 to 8.0
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab134757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionNegative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.
Sequence similaritiesContains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
- DKFZp564K112 antibody
- HDCMA18P antibody
- La related protein 7 antibody
All lanes : Anti-LARP7 antibody (ab134757) at 0.1 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from 293T cells at 50 µg
Lane 4 : Whole cell
lysate from Jurkat cells at 50 µg
Lane 5 : Whole cell
lysate from mouse NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 30 seconds
Gel concentration 4-20%
Lane 1: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of LARP7.
Lane 2: Detection of Human LARP7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134757 at 6 µg/mg lysate for IP.
Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab134757 at 1 µg/ml.
Gel concentration 4-20%
Developed using the ECL technique with an exposure time of 10 seconds.
ab134757 has not yet been referenced specifically in any publications.