Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
Belongs to the LAS1 family.
Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Localizes mainly to the granular component, the region implicated in the later steps of rRNA processing and subunit assembly and export.
Detection of LAS1L in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab190390 at 6 µg/reaction for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3o seconds.