Overview

  • Product name

    Anti-LAS1L antibody [EPR8988(B)]
    See all LAS1L primary antibodies
  • Description

    Rabbit monoclonal [EPR8988(B)] to LAS1L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LAS1L aa 200-300. The exact sequence is proprietary.
    Database link: Q9Y4W2

  • Positive control

    • 293T, HeLa and PC-3 cell lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8988(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
    • Sequence similarities

      Belongs to the LAS1 family.
    • Cellular localization

      Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Localizes mainly to the granular component, the region implicated in the later steps of rRNA processing and subunit assembly and export.
    • Information by UniProt
    • Database links

    • Alternative names

      • dJ475B7.2 antibody
      • FLJ12525 antibody
      • FLJ30133 antibody
      • FLJ45274 antibody
      • LAS1 like (S. cerevisiae) antibody
      • Las1 like antibody
      • LAS1L antibody
      • LAS1L_HUMAN antibody
      • Protein LAS1 homolog antibody
      • Ribosomal biogenesis protein LAS1L antibody
      • RP3 475B7.2 antibody
      see all

    Images

    • All lanes : Anti-LAS1L antibody [EPR8988(B)] (ab140656) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : PC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 83 kDa
      Observed band size: 83 kDa

    • Immunofluorescent analysis of HeLa cells labelling LAS1L with ab140656 at 1/250 dilution.

    References

    This product has been referenced in:

    • Crow MS & Cristea IM Human Antiviral Protein IFIX Suppresses Viral Gene Expression during Herpes Simplex Virus 1 (HSV-1) Infection and Is Counteracted by Virus-induced Proteasomal Degradation. Mol Cell Proteomics 16:S200-S214 (2017). Read more (PubMed: 28077445) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab140656.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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