Product nameAnti-LASP1 antibody
See all LASP1 primary antibodies
DescriptionRabbit polyclonal to LASP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide, corresponding to a region within amino acids 100-150 of Human LASP1 (NP_006139.1).
- Whole cell lysate from HeLa, 293T and Jurkat cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab117806 was affinity purified using an epitope specific to LASP1 immobilized on a solid support .
Our Abpromise guarantee covers the use of ab117806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain.
Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.
- Information by UniProt
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
All lanes : Anti-LASP1 antibody (ab117806) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 30 seconds
ab117806 at 1 µg/ml staining LASP1 by WB following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab117806 at 6 µg/mg lysate.
Lane 2; IP using an antibody to a downstream epitope.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.
This product has been referenced in:
- Li W et al. Long non-coding RNA LINC00672 contributes to p53 protein-mediated gene suppression and promotes endometrial cancer chemosensitivity. J Biol Chem 292:5801-5813 (2017). Read more (PubMed: 28232485) »
- Hu JJ et al. Proteomic analysis on effectors involved in BMP-2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells. Proteome Sci 12:13 (2014). WB ; Dog . Read more (PubMed: 24580839) »