Overview

  • Product name
    Anti-LASP1 antibody [EPR11221]
    See all LASP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11221] to LASP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human LASP1 (C terminal).

  • Positive control
    • Recombinant Human LASP1 protein (ab114888) can be used as a positive control in WB. SK-OV-3, A431, U87-MG and 293T cell lysates, and A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
    • Sequence similarities
      Contains 1 LIM zinc-binding domain.
      Contains 2 nebulin repeats.
      Contains 1 SH3 domain.
    • Cellular localization
      Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • LASP 1 antibody
      • LASP-1 antibody
      • LASP1 antibody
      • LASP1_HUMAN antibody
      • LIM and SH3 domain protein 1 antibody
      • LIM and SH3 protein 1 antibody
      • Metastatic lymph node gene 50 protein antibody
      • MLN 50 antibody
      • MLN50 antibody
      • OTTHUMP00000164238 antibody
      • OTTHUMP00000164239 antibody
      see all

    Images

    • All lanes : Anti-LASP1 antibody [EPR11221] (ab156872) at 1/10000 dilution

      Lane 1 : SK-OV-3 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : U87-MG cell lysates
      Lane 4 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 30 kDa

    • Immunofluorescent analysis of A431 cells labeling LASP1 with ab156872 at 1/100.

    References

    This product has been referenced in:
    • Balahmar RM  et al. Identification and characterisation of NANOG+/ OCT-4high/SOX2+ doxorubicin-resistant stem-like cells from transformed trophoblastic cell lines. Oncotarget 9:7054-7065 (2018). Read more (PubMed: 29467949) »
    • Zhao H  et al. LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway. Oncol Lett 15:6839-6844 (2018). Read more (PubMed: 29731863) »
    See all 3 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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